超高亮度紅、藍光LED應用於蝴蝶蘭組培苗栽培之研究

Using Super-Bright Red and Blue Led in the Production of Phalaenopsis Plantlets in Vitro

饒瑞佶(Ruey-Chi Jao) ； 方煒(Wei Fang) ； 蔡田龍(Tien-Lung Tsai)

《農業機械學刊》 12卷1~4期 (2003/03) Pp. 93-100

https://doi.org/10.30062/JAM.200303.0008

摘要

本研究以自行研發的超高亮度紅光與藍光發光二極體(LED)燈具為人工光源，透過驅動器調整其光量與光質，配合兩種允許上方透光的組培容器進行蝴蝶蘭組培苗的栽培試驗，旨在探討應用此種新型光源（以LEDSet稱之）與使用傳統的螢光燈管在蝴蝶蘭組培苗栽培上的差異。90天的試驗結果顯示兩者在濕重、乾重、葉片數、葉寬、根數及根長上並無差異，但在葉長上則以LEDSet為光源者較長。LED具有體積小、壽命長、耗電小、衰減少、波長固定、幾乎不發熱及使用直流電等優點，目前藍光LED的單價雖仍偏高，但在不遠的將來，在組培環控室中使用LED作為新的人工光源應是指日可待。本研究證實LED可用來栽培蝴蝶蘭組培苗，亦適用於做為光型態發生基礎研究之人工光源。

關鍵字

發光二極體 ； 組織培養 ； 蝴蝶蘭

並列摘要

A light mounting fixture using super-bright red and blue LEDs were used as the artificial light source for the production of Phalaenopsis plantlets in vitro. With the help of a driver, the amount of red and blue light can be adjusted separately, thus, providing the capability of adjusting quantity and quality of light. In this study, the focus was on the feasibility of growing Phalaenopsis plantlets in vitro using this new light source (named LEDSet). After 90 days of experiment, we found no significant differences between LEDs and tubular fluorescent lamps (TFL) on wet/dry weight, number/width of leaves, number/length of roots. However, significant difference was found on the length of leaves. The leaves of plants grown under LEDSets are longer than the control group using TFL. The advantages of using LEDs including: small in size, long usable life, low degradation, low electricity consumption, fixed wavelength, very few heat generated and use direct current as power source. The only drawback is the cost. It is generally believed that the cost will be reduced a lot in the near future. Eventually, using LEDs as the light source for the growth of tissue culture plantlets in an environmental controlled chamber will be cost effective. In general, this study proves that using LEDSets as light source for the production of Phalaenopsis plantlets in vitro is feasible. Also, LEDSets can be used as the light source for the study of photomorphogenesis of plants.

並列關鍵字

Light emitting diodes ； Tissue culture ； Phalaenopsis