Molecular Identification of Uromyces appendiculatus and Uromyces vignae from Taiwan with PCR-based Method

菜豆銹病菌(Uromyces appendiculatus)與豇豆銹病菌(U.vignae)所引起的豆科銹病爲世界性重要病害，然過去由於此種銹菌之形態學與寄主範圍的混淆不清，因而增加此兩種銹病菌在鑑定上的困難。本研究利用核糖體基因之內轉錄間隔區(Internal transcribed spacer, ITS rDNA)的多樣性設計四個專一性引子，可快速且正確的鑑定台灣産菜豆銹病菌與豇豆銹病。其中UA-ITSF與UA-ITSR引子，對菜豆銹豆病菌具有非常高的專一性；而UV-ITSF與UA-ITSR引子，則對豇荳銹病菌具非常高專一性。此外，本研究中所設計的四個引子，對八個來自非豆科作物上的銹病菌均無法增幅到任何DNA條帶。

關鍵字

菜豆銹病菌；豇豆銹病菌；菜豆；豇豆

並列摘要

Bean rust caused by Uromyces appendiculatus and cowpea rust caused by U. vignea are important diseases of these corps in the world. Identification of these two Uromyces species has always been difficult in the past due to confusion in morphological feature and host range of the pathogens. In this study, four primers designed form the internal transcribed spacer (ITS) region of nuclear ribosomal DNA were developed for rapid and reliable identification of the two rust pathogens form Taiwan. Two of the primers, UA-ITSF and UA-ITSR, were highly specific to U. appendiculatus on common bean and the others two primers, UV-ITSF and UV-ITSR, were highly specific to U. vignae on cowpea. The four primers failed to amplify any of the eight other rust pathogens isolated from non-legume crops.

並列關鍵字

Uromyces appendiculatus ； U. vignae ； bean ； cowpea

國際替代計量

Molecular Identification of Uromyces appendiculatus and Uromyces vignae from Taiwan with PCR-based Method

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