紫錐菊組織培養苗體細胞變異之RAPD分析

RAPD Analysis of Somaclonal Variation of In Vitro Echinacea Plantlets

莊淑貞(Su-Jean Chuang) ； 陳駿季(Junne-Jih Chen) ； 周煒裕(Wei-Yu Chou) ； 陳宗禮(Chung-Li Chen) ； 宋濟民(Jih-Min Sung)

《作物、環境與生物資訊》 5卷2期 (2008/06) Pp. 85-92

https://doi.org/10.30061/CEB.200806.0001

摘要

本研究利用逢機增幅多型性核酸技術(random amplification of polymorphic DNA; RAPD)偵測紫錐菊Echinacea purpurea由葉片微體繁殖再生植株之體細胞變異，以10組RAPD逢機引子檢定6組包含母株的微體繁殖組共56個單株個體。結果顯示，10組RAPD逢機引子中4組表現多型性，其多型性條帶分別為UBC-44-1 kb、UBC-52-500 bp、UBC-58-500 bp及Operon-X3-750 bp。進一步利用條帶有無計算遺傳相似係數，其中由母株D3-9繁殖的10個再生植株中，有3個再生植株缺失這些條帶，這3個再生植株與母株的遺傳相似係數為0.926，由D3-9繁殖的其餘7個再生植株與母株的遺傳相似係數均為1。其餘由篩選母株T6-28、T3-23、T5-9、T2-15及D7-4繁殖衍生的40個再生植株與其母株的遺傳相似係數均為1.00，這5組微體繁殖組皆無體細胞變異。

關鍵字

紫錐菊 ； 藥用保健植物 ； 組織培養 ； 體細胞變異 ； 逢機增幅多型性核酸

並列摘要

In the present study, random amplification of polymorphic DNA (RAPD) analysis was used to assess DNA variation among Echinacea purpurea plantlets regenerated from leaf organogenesis. Fifty-six regenerated plantlets, including donor plants, from six different groups were used for RAPD analysis using ten arbitrary primers. The results indicated that polymorphic profiles were detectable from 4 out of 10 primers, which were UBC-44-1 kb, UBC-52-500 bp, UBC-58-500 bp and Operon-X3-750 bp. The calculated coefficients of genetic similarity from those polymorphic profiles indicated that 3 out of 10 regenerated plantlets from donor plant D3-9 showed somalclonal variations. The similarity coefficients for these 3 plantlets and other 7 plantlets were 0.926 and 1.00, respectively. The 40 plantlets from donor plants T6-28, T3-23, T5-9, T2-15 and D7-4 showed no detectable somaclonal variation with similarity coefficients of 1.00.

並列關鍵字

Echinacea purpurea ； Medicinal plant ； Micro-propagation ； Somaclonal variation ； RAPD