This study was to investigate second, fourth and sixth grade children's use of singing voice, and examine the variables of age and gender that influence their use of singing voice. It is a follow-up study of the one that investigated first, third and fifth grade children's singing voice conducted by Ming-Jen Chuang in 2007. The goal of this study is continuous and extensive for children singing voice research in Taiwan. In addition, the Children Singing Voice Measure is the instrument in this study. The study found that girls' use of singing voice is better than that of boys. Approximately, 70% participant children do not properly use their singing voice as they sing.