The purpose of this study is to test the relationship between supervisor's leadership effectiveness and subordinators' job motivation. The job characteristics are defined as moderating variables. Data for this study were obtained questionnaires from business employees. There are five findings are summarized as follows: 1. The influences of supervisor's leadership effectiveness on subordinators' job motivation, intrinsic and extrinsic motivation are positive. 2. The influences of leadership satisfaction and the degree of achievement on subordinators' intrinsic and extrinsic motivation are positive. 3. The influence of supervisor's leadership effectiveness on subordinators' extrinsic motivation is significance for subordinators which have higher task significance and skill variety. 4. The influence of supervisor's leadership effectiveness on subordinators' job and intrinsic motivation is significance for subordinators which have lower task significance and skill variety. 5. The higher subordinators' position is, the higher intrinsic motivation is.