This study will be investigated through physical activity and subjective well-being, to analyze the interaction of physical and mental feelings of Taiwan's university students. The objects are collected from Shih-Chien university Kaohsiung campus by proportional quota sampling, which will be cutting into two groups, heavy exercised, rarely exercise, and the total sample is 105. First, we used distinguish clustering analysis to verify its accuracy, and use T test to differentiate the two groups of subjective well-being. This study found that the average of college students' happiness index is -0.78, it means that college students are not happy under the pressure of schoolwork and school life. In exercise behavior clustering happiness level index test has no significant difference. It means that even the more positive group, they can't pull out a significant gap in happiness index.