高效能液相層析法測定市售化妝品之防曬成分

Analysis of Sunscreen Agents in Cosmetic Products by High Performance Liquid Chromatography

徐照程(Jaw-Cherng Hsu) ； 黃勤婷(Chin-Ting Huang) ； 張嘉芳(Jia-Fang Jang) ； 林曉芳(Sheau-Fang Lin)

《弘光學報》 42期 (2003/11) Pp. 153-158

https://doi.org/10.6615/HAR.200311.42.17

摘要

本研究開發出一種快速準確之HPLC分析法，對防曬化妝品中有效成分含量之檢測工作，具有高度應用價值。採用C_(18)管柱配合甲醇-水(88:12，v/v)含1%(v/v)醋酸等位(isocratic)沖提，可在11分鐘內有效分離五種化學防曬劑，平均理論板數達36440/m，沖提次序為：phenylbenzimidazolesulfonic acid、benzophenone-3、octyldimethyl PABA、octylmethoxycinnamate、butylmethoxylbenzoylmethane。五次重複分析的R.S.D.值介於0.93-1.32間。於濃度1-100ppm範圍線性良好，r^2介方合0.9998-0.9994間，偵測極限可達10-18 ppb。選擇十六種市售防曬化妝品進行分析，發現octylmethoxycinnamate及benzophenone-3的採用率最高，分別為87.5%及43.8%，octyldimethyl PABA在本研究所選擇之所街市售產品中皆未偵測到其存在。

關鍵字

高效能液相層析法 ； 化學性防曬劑 ； 化妝品分析

並列摘要

A rapid HPLC procedure for the analysis of five of the most common sunscreen agents (Phenyl benzimidazole sulfonic acid Benzophenone-3 Octyl Dimethyl PABA Octyl Methoxy cinnamate Butyl Methoxylbenzoylmethane) from cosmetic products is described. Separations were performed on a C18 column eluted with methanol-water (88:12, v/v) containing 1%(v/v) acetic acid under isocratic conditions, and completed in 11 min. with an efficiency of about 36440/m theoretical plate numbers. It offers good reproducibility (R.S.D.=0.93-1.32) and shown to be linear in the range from 1 to 100 ppm. Detection limits range from 10 to 18 ppb with 5 L sample volume. Five sunscreen agents were assayed by applying the method to 16 commercial cosmetic products. The results indicate that Octyl methoxycinnamate (87.5%) and Benzophenone-3 (43.8%) were the most common sunscreen agents, and Octyl Dimethyl PABA was not existed in these commercial cosmetic products.

並列關鍵字

HPLC ； Sunscreen agents ； Cosmetic analysis

參考文獻

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