榕樹透翅毒蛾軟化症病毒之研究

Studies on the Infectious Flacherie of Perina nuda (Fabricius)

王重雄(Chung-Hsiung Wang) ； 吳治宇(Chih-Yu Wu) ； 陳瑋芸(Wei-Yung Chen) ； 陳智維(Chih-Wei Chen)

《中華昆蟲》 18卷4期 (1998/12) Pp. vi+259-271

https://doi.org/10.6660/TESFE.1998025

摘要

實驗室飼育之榕樹透翅毒蛾（Perina nuda）幼蟲發生大量死亡，瀕死幼蟲呈現類似家蠶軟化症（flacherie）之病徵。從呈軟化症之榕樹透翅毒蛾幼蟲可以分離到兩種病毒，一種是榕樹透翅毒蛾核多角體病毒（Perina nuda nucleopolyhedrovirus, PenuNPV），另一種是小病毒。在負染法處理之下，此小病毒呈現正二十面體的對稱，直徑約為30 nm，不具有被膜（envelope）和明顯的表面構造，屬小RNA病毒群種類，命名為PnPV。混合感染PenuNPV之軟化症幼蟲，其血淋巴含有或不含有游離的多角體。經口感染實驗證實小病毒之病原性並不強，但可能對PenuNPV殺蟲而言有協力作用，而對利用幼蟲量產多角體上則有負面的影響。

關鍵字

榕樹透翅毒蛾 ； 軟化症 ； 核多角體病毒 ； 昆蟲 ； 小RNA病毒

並列摘要

Recently, disease outbreaks had caused mass mortalities of laboratoryreared larvae of the ficus transparent wing moth, Perina nuda (Fabricius). Moribund larvae exhibited a syndrome similar to silkworms infected with infectious flacherie virus. Two viruses, P. nuda nucleopolyhedrovirus (PenuNPV) and a new picorna-Iike virus (named PnPV), have been isolated from infected larvae with flacherie disease. Negatively stained preparations showed that the virus exhibits icosahedral symmetry and measures 30 nm in diameter with no envelope and no distinct surface structures. The hemolymph of moribund larvae with flacherie disease is either with or without free polyhedra caused by the infection of PenuNPV, The oral-infection experiments showed that pathogenesis of the picorna-Iike virus is not obvious, but it may cause a synergistic effect in mortality and decrease the yield of polyhedra in infected P. nuda larvae with PenuNPV.

並列關鍵字

Perina nuda ； flacherie ； PenuNPV ； insect ； picorna-Iike virus.