臺灣地區煙草粉蝨（Bemisia tabaci）族群內共生菌之初探

Studies on the Secondary Endosymbionts of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Taiwan

洪永昌(Yung-Chang Hung) ； 柯俊成(Chiun-Cheng Ko) ； 王重雄(Chung-Hsiung Wang)

《台灣昆蟲》 25卷3期 (2005/09) Pp. ii+145-157

https://doi.org/10.6661/TESFE.2005015

摘要

本研究對於臺灣地區所採集之202筆煙草粉蝨族群，利用PCR反應對於煙草粉蝨二級內共生菌之16S rDNA及Wolbachia之表面蛋白基因（Wolbachia surface protein, wsp）的增幅，以進行內共生菌的偵測；結果顯示，臺灣地區55.4%的煙草粉蝨族群受二級內共生菌的感染，38.1%的族群受Wolbachia的感染，再經由類緣關係分析，感染臺灣地區煙草粉蝨之二級內共生菌皆為Proteobacteria γ-3亞綱的種類，而Wolbachia則屬於B群的Con/Rug亞群的種類。由各生物小種感染之比例顯示，此二類內共生菌之分布與生物小種有較密切的相關，然而影響其分布的因素仍需後續的研究以進一步釐清。

關鍵字

粉蝨科 ； 煙草粉蝨 ； 二級內共生菌 ； 生物小種 ； 臺灣

並列摘要

The 16S ribosomal DNA (rDNA) of the secondary endosymbiont and Wolbachia-specific gene, wsp (cell surface protein of Wolbachia), were amplified and sequenced. Based on the occurrence of 16S rDNA and wsp by PCR, more than 55% and 38% of examined B. tabaci populations (n = 202) harbored secondary endosymbiont and Wolbachia, respectively. Molecular phylogenetic analysis of 16S rDNA sequences showed that the secondary endosymbiont was located in the γ-3 clade of a subdivision of the Proteobacteria. Wsp gene sequences revealed that the Wolbachia found in B. tabaci populations of Taiwan belong to group B and subgroup Con/Rug. The results also showed that the carrying/infection rate of secondary endosymbiont/Wolbachia might be related to the biotype of B. tabaci.

並列關鍵字

Aleyrodidae ； Bemisia tabaci ； secondary endosymbionts ； biotype ； Taiwan