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飼糧添加板藍根複方中藥草對土雞生長性能及免疫反應之影響

Effects of dietary supplementation of Isatis indigotica root compound prescription on the growth performance and immune response of Taiwan native chicken

摘要


本試驗在探討飼料中添加板藍根複方中藥草(主要原料:板藍根、金銀花、蒲公英、黃岑、甘草與黨參所組成)對土雞生長性能及免疫反應之影響。本試驗採用1日齡畜試土雞360隻,公母各半,平均分配於4個飼糧處理組,分別為(1)對照組:基礎飼糧(未添加任何抗生素與中藥草);及基礎飼糧額外添加(2)0.5%板藍根等複方粉末;(3)1%板藍根等複方粉末;(4)2%板藍根等複方粉末。飼糧營養成分依畜產試驗所土雞營養需要量手冊配製,每處理組3重複,每重複30隻,公母各半,飼養期間為16週。試驗期間,每隔4週秤取個別雞隻重量,及記錄飼料採食量,計算飼料效率、存活率、收集第8、第16週齡血清抗體力價及第16週齡血清IgA、IgG,並於試驗結束時作腸道絨毛型態觀察。試驗結果顯示,飼糧添加板藍根等複方粉末對畜試土雞之攝食量、飼料轉換率與存活率雖無顯著差異,但8-16週齡之體增重以0.5%板藍根等複方粉末處理組顯著高於對照組(P<0.05);1%板藍根等複方粉末處理組0-16週齡存活率78%高於對照組72%。在空腸絨毛長度方面,以0.5%板藍根等複方粉末處理組顯著高於對照組(P<0.05),有助於空腸養分吸收。在免疫反應方面,16週齡畜試土雞在新城雞病疫苗(ND)抗體力價,以0.5%與2%板藍根等複方粉末處理組顯著高於對照組(P<0.05)。傳染性華氏囊炎(IBD)抗體力價以1%板藍根等複方粉末處理組顯著高於對照組(P<0.05)。免疫球蛋白IgA與IgG隨板藍根等複方粉末劑量增加(0-2%)而有增加趨勢,且高於對照組。

並列摘要


The experiment was to investigate the effects of diets added with Isatis indigotica root compound prescription (main ingredient are Isatis indigotica root, Lonicera japonica Thumb., Taraxacum mongolicum, Scutellaria baicalensis Georgi, Glycyrrhiza uralensis Fisch., Codonopsis pilosulae Radix) on the growth performance and immune response of native chickens. Three hundred and sixty day-old chicks were divided into 4 treated groups. Group 1 was control group without adding antibiotics and Chinese herbs. Group 2, Group 3 and Group 4 were supplemented with 0.5%, 1% and 2% Isatis indigotica root compound prescription, respectively. The diets were formulated according to the manual of nutrient requirement of native chicken of Livestock Research Institute. Each treatment had three replicates and each replicate had 30 birds with half males and half females. Body weight, feed intake, feed efficiency and livability were determined every 4 weeks. Antibody titer at 8 and 16 weeks of age, serum IgA and IgG at 16 weeks of age were measured. At the end of this experiment, types of intestine villi were recorded. The results indicated that although feed added with Isatis indigotica root compound prescription had no significant difference on feed intake, feed conversion ratio and livability, treatment groups added with 0.5% Isatis indigotica root compound prescription had higher body weight gain than the control group at 8-16 weeks of age (P < 0.05). Livability of 1% Isatis indigotica root compound prescription group was higher than that of the control group (78% vs. 72%) during 0-16 weeks of age. In jejunum villus length, 0.5% Isatis indigotica root compound prescription group was significantly longer than the control group (P < 0.05). In immune response, 0.5% and 2% Isatis indigotica root compound prescription group had significantly higher ND antibody titer than the control group (P < 0.05) at 16 weeks of age. 1% Isatis indigotica root compound prescription group had significantly higher IBD antibody titer than the control group (P < 0.05). In immunoglobulin, IgA and IgG increased as the increase of Isatis indigotica root compound prescription (0-2%) and were higher than the control group.

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