添加抗生素對肉雞腸道免疫之影響

本試驗探討飼糧中添加不同抗生素對肉雞腸道免疫能力之影響。使用192隻1日齡愛拔益加肉雛雞，逢機分為對照組（control）、枯草菌素（bacitracin）55 ppm、六肽黴素（nosiheptide）2.5 ppm及羥四環黴素（oxytetracycline, OTC）55 ppm共四組。於3週與6週時，進行腸道免疫之測定。結果顯示在腸道免疫功能上，利用西方點墨方法發現，3週齡雞隻迴腸的溶菌酶含量在六肽黴素組中較對照組為低（P < 0.05），枯草菌素及羥四環黴素處理組則與對照組無顯著差異。添加三種抗生素對雞隻迴腸中T淋巴細胞γδ受體（γδ T cell receptor）的含量及脾臟淋巴球增生反應無顯著影響。添加六肽黴素組於6週齡時，顯著減少血液中IgA含量（P < 0.05），對枯草菌素及羥四環黴素則無顯著影響。添加三種抗生素對3與6週齡雞隻血液中IgG與IgM與對照組相比無顯著影響。綜上所述，不同的抗生素對於免疫調控模式可能不一致，雞隻飼糧添加六肽黴素可能通過改變溶菌酶及IgA來調節雞隻的免疫反應，因飼糧中可添加抗生素種類業已限縮，應積極開發具類似抑菌功能之飼料添加物以替代抗生素。

關鍵字

抗生素；肉雞；腸道免疫； T淋巴細胞γδ受體

並列摘要

This study was to investigate the effect of antibiotics on intestinal immune response. One hundred and ninety-two broilers were randomly allocated to 4 treatments with 4 replicates of 12 birds each. The 4 treatments were: control, bacitracin 55 ppm, nosiheptide 2.5 ppm, and oxytetracycline 55 ppm supplements. Intestinal mucosal immunity was measured at 3 and 6 wk of age. In the intestinal mucosal immunity, the nosiheptide supplemented group significantly decreased the expression of mucosal antimicrobial lysozyme level at 3 weeks of age (P < 0.05), but had no effect on the bacitracin and oxytetracycline groups. The three antibiotic supplements had no significant differences in T cell receptor γδ, ileum and lymphocyte proliferation of blood. The nosiheptide supplementation group had lower blood IgA levels (P < 0.05) at 6 wk of age, but did not affect the bacitracin and oxytetracycline groups. Antibiotic administration did not alter plasma IgG and IgM at 6 wk of age. In conclusion, different antibiotics may be inconsistent in immune regulation. Supplementation of nosiheptide in chicken diets modulates the immune response of chickens by downregulating lysozyme and IgA.

並列關鍵字

Antibiotics ； Broilers ； Intestinal immunity ； γδ T cell receptor

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