射頻生物感測器之研發-應用於蛋白質與核酸檢測

Development of a Radio-frequency Biosensor with Amplified Gold Nanoparticles for Protein and DNA Detection

錢睿宏(Jui-Hung Chien)

指導教授 ： 陳炳煇

國立臺灣大學/工學院/機械工程學研究所/博士(2007年)

https://doi.org/10.6342/NTU.2007.00604

摘要

本論文主要提出一新式的生物感測器,此生物檢測系統的主要原理是利用射頻共面波導的電路，並且在電路結構中植入奈米材料的共軛高分子，當病原體的核酸序列進入該共軛結構之後，會連接奈米金顆粒，進而改變射頻共面波導的電磁行為，達到檢測的目的。實驗結果顯示該感測器在低濃度的DNA或是蛋白質的檢測中均能達到良好的結果。

關鍵字

射頻 ； 奈米金

並列摘要

This study presents a novel detection method for protein and DNA with multi-layer AuNPs to enhance overall detection sensitivity. Since the electromagnetic behaviors of the sensors can be altered by utilizing the existence of multilayer AuNPs in the biosensor, the change of the bandwidth of the sensing circuits can be the critical factor in evaluating the performance of the proposed biosensor.
The measured 3-dB bandwidth of the original biosensor is about 18.8 GHz, which is close to the simulated results. The change in bandwidth for multi-layer AuNPs, which is formed by complementary target DNA, exceeds that of the double-layer AuNPs by 0.5 GHz. The changes of the 3-dB bandwidth are proportional to the logarithmic values of the analyte concentrations. Furthermore, the detection limit of the developed biosensor for the DNA set in this essay is 10pM, while it is 1ng/μL for the protein in this essay. In addition, with the temperature controlling system, single mutation of target DNA could be distinguished as the tested target DNA with an approximated length of 27 bps.
The experimental results in this study indicate that this biosensor and chemical detection methodology are successful. As health care becomes much more essential in modern life, this biosensor has potential applications in a screening kit for recognizing, sensing, and quantifying biomolecules in real samples.

並列關鍵字

RF ； AuNPs

參考文獻

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Bergveld, P., 1991, “A Critical Evaluation of Direct Electrical Protein Detection Methods,” Biosens. Bioelectron., 6, pp. 55-72.

Bemey, H., West, J., Haefele, E., Alderman, J., Lane, W, and Collins, J. K., 2000, “A DNA Diagnostic Biosensor: development, Characterisation and Performance”, Sens. Actuators B, 68, pp. 100-108.

Braun, B., Eichen, Y., Sivan, U., and Ben-Yoseph, G, 1998, “DNA-Templated Assembly and Electrode Attachment of A Conducting Silver Wire,” Nature, 391, pp. 775-778.

Brust, M., Bethell, D., Kiely, C. J., and Schifflin, D. J., 1998, “Self-Assembly of Gold Nanoparticle Thin Film with Nonmetallic Optical and Electronic Properties,” Langmuir, 14, pp. 5425-5429.