- 學位論文
香瓜茄水萃物對於以L-NAME誘發高血壓小鼠之影響
The effect of SolanummuricatumAit extract on L-NAME-induced hypertension in mice
摘要
近年來，心血管疾病已成為許多國家中主要死亡原因之一，又因高血壓為心血管及腦血管疾病之危險因子，故高血壓已經成為國人致命的慢性疾病之一。香瓜茄為茄科茄屬草本植物，為台灣澎湖地區特有的水果。在當地相傳香瓜茄具有降低血壓及控制糖尿病之效益，但目前與其相關的科學文獻仍然不多。至於香瓜茄是否具有降低血壓的作用仍是一值得探討之課題。因此，本實驗以一Nω-Nitro-L-arginine methyl ester- hydrochloride (L-NAME)誘發ICR小鼠高血壓，以1%香瓜茄水萃物餵食10週後犧牲，觀察小鼠之血壓改善的情形。結果顯示，餵食香瓜茄水萃物的組別與高血壓組相比，收縮壓及舒張壓皆有顯著的改善；血管收縮素轉化酶(ACE)的蛋白表現量、醛固酮(Aldosterone)及血管加壓素(arginine vasopressin)含量皆顯著下降；且鈉離子依賴型葡萄糖運輸蛋白(SGLT1)及上皮細胞鈉離子通道(ENaC)的蛋白表現量也皆有顯著下降的趨勢；在抗氧化方面，腎臟中的抗氧化酵素活性皆顯著增加，能降低氧化傷害；與體內發炎反應相關的細胞激素TNF-α、IL-1β、IL-6亦有顯著下降。綜合上述結果得知，香瓜茄水萃物的給予是具有改善高血壓並降低發炎反應及增加抗氧化能力的效果。
並列摘要
Cardiovascular disease is one of the main causes of death in many countries including Taiwan. Hypertension is the most common risk factor responsible for the progression of cardiovascular and cerebrovascular diseases. Solanum muricatum Ait (pepino) is a popular fruit in Penghu, Taiwan. Residents of Penghu consider that pepino could reduce blood pressure and control diabetes, but the scientific literature associated with anti-hypertensive effects of pepino was less. Thus, we used L-NAME to induce hypertension in ICR mice and investigated the protective effects of pepino aqueous extract. Mice were fed by 1% aqueous pepino extract for ten weeks. Blood and tissues were collected to analyze. Results showed that the intake of pepino extract significantly improved systolic blood pressure and diastolic blood pressure of mice. Furthermore, this extract significantly reduced the protein expression of ACE , aldosterone and arginine vasopressin concentration. The protein expression of ENaC and SGLT1 was also significantly decreased. In addition, this extract significantly increased glutathione content, catalase and glutathione peroxidase activities, as well as total antioxidant capacity in kidney. Pro-inflammatory cytokines such as TNF-α, IL-1β, IL-6 and NF-κB protein expression were also lowered. These results suggested that pepino aqueous extract could attenuate hypertension, and decrease oxidative and inflammatory stress.