香瓜茄水萃物對於STZ誘發糖尿病小鼠紅血球與坐骨神經多元醇路徑之影響

The effects of Solanum muricatum Ait aqueous extract on erythrocytic and sciatic nerve in STZ-induced diabetic mice via regulation of polyol pathway

林美紅(Mei-Hung Lin)

指導教授 ： 徐成金

中山醫學大學/健康管理學院/營養學研究所/碩士(2012年)

https://doi.org/10.6834/CSMU.2012.00114

摘要

糖尿病是一種慢性新陳代謝異常疾病，其中胰島素阻抗所造成的代謝障礙，會造成細胞無法將葡萄糖帶入細胞內產能利用，使得血糖上升。細胞長期處於高血糖狀態下會誘導自由基大量的生成，造成體內氧化壓力上升，促使多餘的葡萄糖走向多元醇路徑代謝，最終易形成糖化終產物，引發糖尿病併發症的產生。
香瓜茄是澎湖特有的農作物，當地居民相傳是可緩和糖尿病的水果，先前本實驗室發現以STZ誘發第1型和第2型糖尿病的BALB/c小鼠餵食香瓜茄，結果顯示，血清、肝臟和腎臟皆可減少脂質過氧化情形且會抑制多元醇路徑中限制酵素-醛醣還原酶的活性，延緩糖尿病併發症的發生，對於以lipopolysaccharide誘發發炎反應的情況下也同樣具有保護作用。
本研究將探討不同甲醇濃度做層析減糖的香瓜茄水萃物，餵食誘發糖尿病BALB/c小鼠，觀察紅血球與小白鼠後腿坐骨神經的體內傷害與多元醇路徑之影響。實驗設計採用streptozotocin誘發糖尿病動物模式(40mg/kg BW/day)，連續五天腹腔注射，分別餵食不同甲醇濃度做層析減糖的香瓜茄水萃物，八週後犧牲，取血清、紅血球和小白鼠後腿坐骨神經進行分析。
結果顯示糖尿病小鼠餵食不同甲醇濃度做層析減糖的香瓜茄水萃物，可減少脂質過氧化情形及多元醇路徑的關鍵酵素-醛醣還原酶活性、產物Sorbitol、Fructose含量和糖化終產物指標CML含量，皆表現量有顯著性下降，由此研究證實香瓜茄水萃物，在紅血球與小白鼠後腿坐骨神經，同樣可延緩糖尿病小鼠併發症的發生。

關鍵字

香瓜茄 ； 糖尿病 ； 氧化壓力 ； 多元醇路徑

並列摘要

Diabetes is a chronic metabolic disorder. Its characteristics include hyperglycemia, hyperinsulinemia, oxidative stress and glycative injury, which also involve the activation of polyol pathway and consequently cause complications. Thus, the intervention of food with anti-oxidative and/or anti-glycative potential may be helpful to alleviate diabetic progression. Pepino (Solanum muricatum Ait ) is a popular fruit in Penghu. Our previously studies have reported that this the intake of this fruit could benefit streptozotocin (STZ)-induced diabetic mice via improving hyperglycemia, hyperlipidemia, oxidative stress and glycative injury in tissues. Thus, our present study was designed to further examine the protective effects of this fruit upon polyol pathway in red blood cell and sciatic nerve from diabetic mice. Aldose reductase (AR) activity and the level of advanced glycative end- products (AGEs) such as N-ɛ-carboxymethyl-lysine (CML) were also determined. Results showed that the intake of pepino aqueous extract at 1% for 8 weeks markedly alleviated insulin resistance in STZ-induced diabetic mice, and also decreased oxidative damage and lowered AR activity, fructose and CML production in erythrocyte and sciatic nerve. These findings suggest that pepino could decline diabetic progression via suppressing polyol pathway and lowering AGEs production. Thus, our data support that pepino is a potent anti-diabetic plant food.

並列關鍵字

Solanum muricatum Ait ； diabetes ； oxidative stress ； polyol pathway

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