香瓜茄水萃物對於STZ誘發第2型糖尿病與LPS誘發發炎反應的動物模式之影響

The effect of an aqueous extract from Solanum muricatum Ait on STZ-induce Type 2 Diabetes and LPS-induce inflammation modle.

巫玫靜(Mei-jing Wu)

指導教授 ： 徐成金

中山醫學大學/健康管理學院/營養學系碩士班/碩士(2011年)

https://doi.org/10.6834/CSMU.2011.00013

摘要

糖尿病是一種慢性新陳代謝疾病，長期處於高血糖狀態下，使得體內氧化壓力增加。過量產生的自由基會造成Glyceraldehyde-6-P的堆積，細胞內糖化終產物的生成增加，高血糖也會促使葡萄糖走向多元醇路徑來代謝多餘的葡萄糖，易導致併發症的產生。香瓜茄(Solanum muricatum Ait )是澎湖特有農作物，先前本實驗室已證實香瓜茄可以延緩第1型糖尿病的併發症，因此本研究將探討香瓜茄水萃物餵食誘發第2型糖尿病BALB/c小鼠，觀察香瓜茄對於延緩糖尿病小鼠體內的傷害與多元醇路徑之影響，以及lipopolysaccharide誘發發炎反應是否也有相同作用。採用以streptozotocin誘發第2型糖尿病動物模式(40 mg/kg BW/day)，連續五天腹腔注射，再分別餵食1%、2%與5%香瓜茄水萃物八週，取血清和組織臟器進行實驗分析。結果顯示糖尿病小鼠餵食不同劑量的香瓜茄水萃物八週後，能改善胰島素阻抗性，降低血清、肝臟、腎臟、胰臟氧化壓力，而腎臟多元醇路徑的關鍵酵素aldose reductase活性和mRNA表現量顯著下降，且血清
fructosamine含量降低。另外，餵食5%香瓜茄水萃物四週後，犧牲前16小時再以lipopolysaccharide (10mg/kg BW)誘發Balb/c小鼠體內發炎反應。結果顯示小鼠餵食香瓜茄水萃物可降低血中發炎指標TNF-α及體內氧化壓力。因此經由研究證實香瓜茄水萃物可降低體內氧化壓力及糖化作用來延緩第2型糖尿病小鼠的併發症病程，對於以lipopolysaccharide誘發發炎反應情況下也同樣具有保護作用。

關鍵字

香瓜茄 ； 第2型糖尿病 ； 多元醇路徑 ； 氧化壓力

並列摘要

Diabetes is a chronic metabolic disease. Hyperglycemic condition enhances oxidative stress, the over-production of free radicals causes accumulation of glyceraldehyde-6-P and advanced glycation end-products. Hyperglycemia also promotes glucose conversion into polyol pathway and developed favors progression of diabetic complications. Solanum muricatum Ait (Pepino) is a popular vegetable in Penghu. Our previous study indicated that the fruit of Pepino was able to improve type 1 diabetes. The purpose of the present study was to investigate the effects of an aqueous extract from Pepino on antioxidant defense and polyol pathway in streptozotocin (STZ)-induce Type 2 Diabetic BALB/c mice. Furthermore, anti-inflammatory effects of this fruit in lipopolysaccharide (LPS)-treated mice were also examined.
Type 2 diabetes was induced by STZ (40 mg/kg BW/day, for five consecutive days). After feeding the aqueous extract from Pepino for 8 weeks, the serum and tissue were collected to analyze. Results showed that Pepino improved insulin resistance, decreased oxidative damage in serum, liver, kidney and pancreas. The activity and mRNA expression of aldose reductase, key enzyme in poly pathway, decreased in kidney and the fructosamine level in serum was also reduced. On the other hand, LPS increased oxidative and inflammatory stress, and the intake of aqueous extract from Pepino reduced the level of TNF-α and oxidative stress. These data suggest that aqueous extract from Pepino could provided anti-oxidative, anti-inflammatory and anti-glycosylative effects.

並列關鍵字

Solanum muricatum Ait ； poly pathway ； oxidative stress

參考文獻

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