- 學位論文
複合式光磁性奈米核殼結構結合生物型標示之製造方法
Manufacturing process by mixing the complex optical magnetic nanoscale nuclear shell structure with the biological labels.
摘要
本文在發展一種具光磁特性之核殼結構，並結合生物相容性之標記而合成出奈米複合顆粒。本研究所發展之核殼結構，其磁性可應用於生醫相關領域，也可以用於特定組織之偵測細胞及輔助顯影功用或應用於標靶藥物治療等方面。以化學沉積法所製備出之純淨Fe3O4奈米磁性流體，其平均粒徑約為25nm，並且於Fe3O4顆粒表面上吸附一層硫化鋅(zinc sulfide)外殼。由顆粒表面電位之檢測結果顯示，Fe3O4顆粒表面帶有正電，因此可增加與寡核苷酸結合之效率。本研究將製備出之核殼奈米複合顆粒與兩組不同序列之寡核苷酸及ㄧ组橋接寡核糖核酸連結，並混合於培養皿中，這三種不同序列之寡核苷酸片段鹼基(base)係設計成部分互補，藉由DNA之間的雜合反應(hybridization)而使之互相連接在一起；並且於反應後分離出未鍵結於Fe3O4/ZnS 核殼顆粒表面之寡核糖核酸片段，以及未發生雜合反應之寡核苷酸，而僅保留已互相連接及產生雜合反應之產物，經由TEM image證實，Fe3O4顆粒係相互緊靠在一起，並進一步分析此核殼結構之形貌；另以UV-vis分光度計分析經雜合反應後的流體中，確定有DNA存在；同時配合染色法報導基因進而驗證出本文所發展之不同型式DNA間的雜合技術乃十分成功。
並列摘要
This paper develops a kind of core-shell structure with optical magnetic properties, and conjugates it with biological labels to synthesize nanocomposite particle. The magnetic properties of the core-shell structure developed by the study can be applied to the related biological and medical domains, cell detection of specific tissue, and the auxiliary development function, or the aspect of target-based drug therapy. For the pure Fe3O4 nanoferrofluid prepared by chemical disposition method, its average particle size is 25nm, and a layer of zinc sulfide shell is absorbed on the surface of Fe3O4 nanoparticles. In order to make Fe3O4 nanoferrofluid keep at a stable suspension and have good dispersion properties, its quality is modified by applying hydrophilic on the particle surface. On the surface of the shell structure is the thiol group, which is available for conjugation with oligonucleotide primer. After that, an absorption bonding is conducted between the oligonucleotide primer. The inspection result of ξ-potentialmeter shows that the surface of Fe3O4 particle carries positive charge, thus increasing the efficiency of its conjugation with oligonucleotide. The study conjugates the prepared Fe3O4/ZnS core-shell nanocomposite particles with two groups of oligonucleotide and a brigdge oligonucleotide primer with different sequences, and mixes them. These three groups of oligonucleotide primer base with different sequences are designed to be partially complementary. They are made mutually conjugated together through the hybridization of DNAs. Furthermore, TEM and SEM image analyzes the morphology of the core-shell structure. Besides, as analyzed by UV-vis spectrophotometer, it is confirmed that DNA exists in the product fluid after hybridization reaction. Also supported by colormetric reporter gene, the hybridization technology among different sequences of DNA developed by this paper is proved successful .
參考文獻
被引用紀錄
延伸閱讀
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