以QCM探討改質界面對蛋白質吸附影響之研究

To Explore The Modified Interface Effect on Protein Adsorption by QCM

許芳華(Fang-Hua Shiu)

指導教授 ： 陳慶國

國立臺北科技大學/工程學院/化學工程研究所/碩士(2013年)

摘要

本研究以QCM 作為質量感測器，觀察裸金晶片和改質晶片之蛋白質吸附能力。表面改質選用3-mercaptopropionic acid 、 2-mercaptoethanol 及 1-butanethiol，先對金電極表面做化學性的修飾處理。由於碳氫鏈間的凡得瓦力，加上金-硫間的共價鍵結，使得單分子具有緻密排列的特性，在金電極上形成自我聚集單層膜（self-assembled monolayer，SAM）。三種SAM因為末端的官能基不同（烷基、羧酸根和氫氧基），使得電極表面呈現不同親疏水性。蛋白質選擇使用牛血清蛋白（bovine serum albumin，BSA） ，濃度範圍1mM∼0.01mM，共取七種蛋白質濃度，用QCM觀察不同蛋白質濃度與吸附能力之關係。
改質界面也進一步用儀器測定其物性，如以接觸角量測儀測量表面的親疏水性；掃描式電子顯微鏡（scanning electron micriscope，SEM）用來觀察晶片表面的粗糙度。

關鍵字

石英晶體微天平 ； 晶片改質 ； 自我聚集單層薄膜 ； 牛血清蛋白

並列摘要

Using QCM as a quality sensors, research bare gold chips and the other chips are modified protein adsorption capacity. Surface modification selection of 3-mercaptopropionic acid, 2-mercaptoethanol, 1-butanethiol to the surface of the gold electrode chemical modification processing. Due to the van der Waals force between the hydrocarbon chain, with gold - sulfur covalently bound single-molecule has a dense array of features, the gold electric shock on the formation of self-assembled monolayer ( SAM ). The three SAM because the end of the different functional groups (alkyl、carboxylic acids and hydroxyl), making the surface of the electrode showing different hydrophobicity. Protein using bovine serum albumin ( BSA), the concentration range of 1mM ~ 0.01mM（m mol/L）take the seven protein concentration, the relationship between the type of protein concentration and the adsorption capacity was observed with the QCM.
The modified interfaces are further measured their physical properties. Contact angle measuring instrument is using to measuring its hydrophobicity; scanning electron microscopy is empolyd to observe the uniformity of SAM surfaces.

並列關鍵字

QCM ； surface modified ； self-assembled monolayer ； BSA

參考文獻

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