鈦基合金表面生成二氧化鈦薄膜之生物相容性研究

Research of biocompatibility on titanium-based alloy with titanium dioxide film

陳宜妤(Yi-Yu Chen)

指導教授 ： 林哲堂

共同指導教授 ： 歐耿良

臺北醫學大學/口腔醫學院/牙醫學系碩博士班/碩士(2011年)

https://doi.org/10.6831/TMU.2011.00065

摘要

牙科植體漸漸成為治療缺牙區的選項之一時，許多學者便致力於研究植體與組織的交互作用，經由表面形態的改變，追求更有效率的骨整合。已有研究提出鈦金屬表面形成的氧化層跟其優良的生物相容性有關，因此瞭解氧化層的性質與改變氧化層的結構、形態、厚度等，讓人甚感興趣。
本實驗利用電漿處理的表面處理方式，得到不同表面氧化層的鈦金屬試片，以人類骨肉瘤細胞培養後觀察其所呈現的細胞形態以及與試片接觸的情形，同時比較血液相容性的差異。結果顯示經過處理的鈦金屬，其生物相容性與血液相容性都有改善的現象。

關鍵字

鈦金屬 ； 表面處理 ； 牙科植體

並列摘要

The biocompatibility of titanium is closely related with the surface oxide film. The improvement of the biological activity of titanium with plasma immersion on implantation has been investigated. However, the effects of oxygen ion implantation on the titanium were still lack. In this study, the titanium plates were implanted with oxygen at different power conditions and subsequently analyzed for surface morphologies and phase composition. To observe the effect of oxygen ion implantation on cell behavior, MG-63 osteoblast-like cells were cultured on the treated titanium plates. The hemocompatibility was determined by measuring the adhesion of blood platelets and fibrinogen, P-selectine expression, and the blood clotting time on these modified titanium plates. The results revealed that MG-63 osteoblast-like cells expressed better response on the surface-treated titanium plates. Furthermore, the results of clotting time assay demonstrated titanium treated by oxygen ion power could promote blood coagulation. The titanium plates treated with 1 KW ion power revealed better expression of platelets and fibrinogen adhesion, which showed the higher average roughness. In conclusion, the biocompatibility and hemocompatibility of titanium-based alloy can potentially be improved by plasma immersion oxygen ion implantation.

並列關鍵字

titanium ； surface treatment ； dental implant

參考文獻

Advincula, M. C., F. G. Rahemtulla, et al. (2006). "Osteoblast adhesion and matrix mineralization on sol-gel-derived titanium oxide." Biomaterials 27(10): 2201-2212.

Albrektsson, T., P. I. Branemark, et al. (1981). "Osseointegrated titanium implants. Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man." Acta Orthop Scand 52(2): 155-170.

Batzer, R., Y. Liu, et al. (1998). "Prostaglandins mediate the effects of titanium surface roughness on MG63 osteoblast-like cells and alter cell responsiveness to 1 alpha,25-(OH)2D3." J Biomed Mater Res 41(3): 489-496.

Bobyn, J. D., R. M. Pilliar, et al. (1980). "The optimum pore size for the fixation of porous-surfaced metal implants by the ingrowth of bone." Clin Orthop Relat Res(150): 263-270.

Bowers, K. T., J. C. Keller, et al. (1992). "Optimization of surface micromorphology for enhanced osteoblast responses in vitro." Int J Oral Maxillofac Implants 7(3): 302-310.