生育三烯醇對四氯化碳誘導大鼠慢性肝損傷之護肝功效評估

文獻指出生育三烯醇具有抗氧化，抑制脂質過氧化，降低膽固醇，降低凝血因子血栓素B2，及抗癌症等功能。本研究目的為探討油棕櫚(Elaeis guineensis) 果實的生育三烯醇濃縮物(TRF) 對四氯化碳誘導大鼠慢性肝損傷之護肝功效。實驗共分五組，分別為正常控制組（未經四氯化碳處理）、四氯化碳處理負控制組（僅投予四氯化碳）、水飛薊素處理正控制組（四氯化碳與水飛薊素處理）、低劑量(TRF-25) 及高劑量(TRF-50) 樣品處理組（四氯化碳分別與TRF 25mg/kg BW、50mg/kg BW）。結果顯示，經連續八週TRF餵食後，兩種劑量的TRF均能降低四氯化碳誘導肝損傷後血清中之麩丙酮酸轉胺酶與麩草醋酸轉胺酶，提升總抗氧化能力，並提高肝臟的超氧化物歧化酶、過氧化氫酶，麩胱甘肽過氧化酶的濃度與血清中總抗氧化力。本研究推論 TRF藉著提昇抗氧化酶活性、降低肝發炎後血清中麩丙酮酸轉胺酶與麩草醋酸轉胺酶濃度，並減少脂質過氧化來達到護肝之作用。

關鍵字

生育三烯醇；慢性肝損傷；四氯化碳

並列摘要

Studies have shown that tocotrienols possess antioxidant, anti-lipid peroxidation, cholesterol lowering, reducing blood coagulating factor thromboxane B2 and anti-cancer activities. In this study, we examined the hepatoprotective effects of palm tocotrienol rich fraction (TRF) against carbon tetrachloride (CCl4) induced chronic liver damage in rats. Study was divided into five groups, namely groups one and two received normal saline without and with CCl4 respectively. Groups three, four and five received silymarin, TRF 25mg/kg (TRF-25), and TRF 50mg/kg (TRF-50) plus CCl4 respectively. Results showed that after 8 weeks of TRF-25 and TRF-50 treatment, TRF significantly reduced TBARS (thiobarbituric acid reactive substances) , ALT (alanine aminotransferase) and AST (aspartate aminotransferase) levels, while increased the blood total antioxidant capacity, superoxidase dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) concentration in CCl4 induced liver injury groups. In addition, total cholesterol (TC), low-density lipoprotein (LDL) were noted to be significantly decreased in the TRF treated groups. These results suggest that TRF can effectively enhanced the antioxidant enzymes and reduced the level of ALT and AST. Taken together, this study concludes that TRF was able to protect liver against CCl4 induced liver injury in rats through enhancing the levels of antioxidant enzymes and decreasing ALT, AST and lipid peroxidation.

並列關鍵字

tocotrienols ； chronic liver injury ； CCl4

參考文獻

1. 林松洲。2005。各種疾病的自然療法。凱侖出版社，台北。pp1-106。

Google Scholar

2. 楊榮森。2005。臨床營養學-靜脈暨腸道營養。時新出版社，台北。pp 401-36。

Google Scholar

3. Abraham P, Wilfred G, Cathrine SP. 1999. Oxidative damage to the lipids and proteins of the lungs, testis and kidney of rats during carbon tetrachloride intoxication. Clin Chim Acta 289:177-9.