以磁珠為基礎之生醫感測系統開發及應用

Development and Application of Magnetic Bead Based Biosensing System

黃麗錦(KimberlyJaneSih Uy)

指導教授 ： 鍾文耀

中原大學/工學院/電子工程研究所/博士(2014年)

https://doi.org/10.6840/cycu201400158

摘要

近年來，對於較優的醫療照護品質有很大的需求。即時檢測儀器，由於具有方便性與較快的檢測效率，已經成為醫療照護必備的一環。動脈粥樣硬化的疾病是已開發國家的主要死因，內臟脂肪組織的堆積在動脈粥樣硬化疾病的發生，扮演很重要的角色。代謝症候群對於動脈粥樣硬化疾病的形成是一重要的警訊，而代謝症候群可以由內臟脂肪的增加與脂締素的降低來決定。脂締素，是一種蛋白質，存在於內臟脂肪組織。脂締素的濃度通常可以經由酵素免疫分析法來檢測。本研究將探討脂締素及其和代謝症候群與動脈粥樣硬化疾病的關係，本研究開發一套利用磁性粒子披覆鍵結的檢測平台以取代傳統酵素免疫分析法的脂締素檢測。本研究也將進行磁性粒子檢測技術的回顧，在此前提下，本研究實現一自動化微流體系統以進行磁性粒子之免疫分析。此系統的探討也將包括關鍵零組件的功能描述。另一方面，此研究會包含具有霍爾效應的磁性場效電晶體之理論背景探討、元件晶片製作、及實驗結果等，以應用於磁珠免疫分析中磁性粒子定量的檢測。

關鍵字

即時檢測儀器 ； 肥胖 ； 磁性粒子檢測技術 ； 磁珠(磁性粒子) ； 脂締素 ； 磁性場效電晶體

並列摘要

There is a great increase in the need for better health care in recent years. The point of care testing (POCT) devices has become an essential part of health care due to its convenience and rate of testing. Atherosclerotic diseases are the leading cause of death in developed countries. Visceral adipose tissue accumulation plays a major role in the occurrence of atherosclerotic diseases. Metabolic syndrome is claimed to be a danger signal for atherosclerotic disease. The metabolic syndrome can be determined by the increase in visceral fat and decrease in adiponectin level. Adiponectin is a protein that exists in adipose tissues. The levels of adiponectin are usually determined with the use of enzyme linked immunosorbent assay (ELISA). This research will provide a discussion on adiponectin and its connection to metabolic syndrome and atherosclerotic disease. This work presents a system that will make use of magnetic particle assay (MPA) as a means of measuring the level of adiponectin protein in place of ELISA. It will also provide an overview on existing technology for detecting magnetic beads. Within the framework of this research an automated fluid delivery system for the implementation of the MPA developed and used. The descriptions of the system will also contain functional description of its crucial components. In addition, the work will also include theoretical background, chip fabrication as well as experimental result for a Hall effect sensor magnetic field effect transistor (MagFET) which are the sensors to be used in quantifying the amount of magnetic beads after implementation of the MPA process.

並列關鍵字

Obesity ； Point of Care Technology ； Magnetic Beads ； Magnetic Particle Assay ； Adiponectin ； Magnetic Field Effect Transistor

參考文獻

Chapter 1

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