陣列式生醫感測元件與信號讀取電路之研究

Study on Bio-medical Array Sensors and Signal Readout Circuits

謝秀利(Hsiu-Li Shieh)

指導教授 ： 孫台平

國立暨南國際大學/科技學院/電機工程學系/博士(2015年)

摘要

本研究提出了一種差動對之延伸式基極雙載子電晶體( extended base bipolar junction transistor, EBBJT)經實驗驗證成功實現單一感測器之讀出電路，該感測器讀出電路將被用來降低於環境中之共模雜訊以得到延伸式基極雙載子電晶體尿素感測器與pH變化之線性感測特性。
單一尿素生物感測器之檢測範圍於10mM磷酸鹽緩衝溶液中，代測尿素濃度從1.4 mg/dL至64 mg/dL內，該尿素生物感測器之靈敏度基於EBBJT 65.8mV/ pUrea，單一檢測pH之變化測量，於氫離子濃度pH 2至pH 10之間，該延伸式基極雙載子電晶體之輸出響應具有約59.3mV / pH之線性反應。
電壓式陣列型酸鹼感測器，係以二氧化鈦(Titanium dioxide, TiO2)材料為基礎，製作出「1×4陣列型電壓式酸鹼感測器」並就感測器製程與讀出訊號應用做基礎探討，利用真空蒸鍍系統在金導體上沉積二氧化鈦，藉由Al2O3/ Au/ TiO2，印刷金導體表面經由二氧化鈦薄膜修飾後，亦具感測氫離子之特性，以完成陣列型電壓式酸鹼感測器之研製，偵測範圍於 pH3~pH13之間，線性電壓感測器總平均感測度為64.7mV/pH。
陣列式電流式葡萄糖感測器，採用光聚合膠體包埋方法固定酵素，利用紫外光
356nm控制PVA-SbQ之聚合將酵素或去氫脢置於PVA-SbQ之單體中照射感測薄
膜，使其形成聚合膠體，葡萄糖氧化酵素與電子傳遞物固定於金電極表面，完成
電 流式葡萄糖生物感測器之備製，外加電壓設定於 2V、電流設定100uA之條
件下，電流式葡萄糖生物感測器之偵測分別100mg/dl至400mg/dl，線性電流感測
器總平均可獲得感測度為12 uAcm-2/mMol。
最後，陣列式感測元件開發係以三氧化二鋁陶瓷基板為基底，經過塗層與燒結技
術形成絕緣及金體感測薄膜基板，具有抗形變、耐酸蝕及耐高溫之優點，完成生醫
感測器製作，結合適當之讀出電路，即成為一完整的陣列式感測器系統。

關鍵字

基極雙載子電晶體 ； 尿素生物感測器 ； 二氧化鈦薄膜 ； 金電極 ； 陣列型酸鹼感 測器 ； 陣列型葡萄糖感測器

並列摘要

This study proposes an approximation simulation for a differential pair, which is an extended base bipolar junction transistor (EBBJT). We successfully accomplished the reading of a circuit using a single sensor through experiments. The circuit being read by the sensor is used to reduce the common mode noise in the chemical environment to obtain the linear sensor characteristics of the changes in pH as well as the urea sensor of the EBBJT.
The sensitivity of the urea biosensors is based on the EBBJT at 65.8mV/pUrea in terms of the detection range as a single urea biosensor in a 10mM phosphate buffer solution with the testing urea concentration measuring from 1.4 mg/dL to 64 mg/dL.The output response of the EBBJT had a linear response of about 59.3mV/pH and for measuring changes in the single pH detector with the concentration of the hydrogen ions was between pH 2 to pH 10.
The potentiometric pH sensor array used titanium dioxide (TiO2) to produce a 1 × 4 potentiometric pH sensor array. Discussions were based on the production process of the sensor as well as the application of the output signals. We used a vacuum evaporation system to deposit titanium dioxide on the metal conductor, Al2O3/ Au/ TiO2, and printed the characteristic of the metal conductor surface when it had been modified by the titanium dioxide film. The additional ability to sense hydrogen ions completed the development of potentiometric pH sensor array. The sensing range was between pH 3 to pH 13 and the total average sensitivity for the linear voltage sensor sense was 64.7mV/pH.
The amperometric glucose sensor array used gel entrapment with Photo-polymerization to fix the enzymes. Ultraviolet at 356 nm was used to control the polymerization of PVA-SbQ and to place the enzyme or the dehydrogenase in the PVA-SbQ monomer to illuminate the sensing film, which can form a piece of polymeric gel. The glucose oxidase and the electron transport materials were fixed onto the metal electrode surface. This completed the preparation of an amperometric glucose biosensor. The applied voltage was configured at 2V and the current as 100uA. The amperometric glucose biosensor can detect between 100mg/ dl to 400mg/ dl. The total average of the linear current sensor can achieve a sensitivity of 12 uAcm-2 / mMol
Finally, the developed of the array sensing element with substrate based on a ceramic substrate with aluminum oxide. It also used coating and sintering technology for insulation and metal sensing. The substrate has few advantages including resistance to deformation, acid corrosion, and high temperature. This completes the production of a biomedical sensor. It becomes a comprehensive array sensor system when combined with appropriate circuit reading.

並列關鍵字

base bipolar junction transistor ； urea biosensors ； Titanium dioxide ； Au electrode ； array pH sensing ； array glucose sensing

參考文獻

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