利用幾丁聚醣與藻酸鈉包埋益生菌枯草桿菌E20 以改善白蝦成長及健康之效率

Chitosan coated alginate encapsulated Bacillus subtilis E20 improves the growth and health of white shrimp, Litopenaeus vannamei

盧希塔(Adilah Rusyda Nur)

指導教授 ： 劉俊宏 ； 胡紹揚

國立屏東科技大學/農學院/生物科技系/碩士(2022年)

https://doi.org/10.6346/NPUST202200076

摘要

本研究旨在將益生菌枯草芽孢桿菌E20微膠囊化包裹在藻酸鹽與幾丁聚醣中，評估其在模擬胃液(SGF) pH 2、模擬腸液 (SIF) pH 7 中的活力，並評估包埋益生菌 B. subtilis E20 在不同劑量下對蝦生長性能和抗病性的影響。以濃度為 1.5% 和 2% 的藻酸鹽包埋的枯草芽孢桿菌 E20 在 SGF 和 SIF 中的存活率顯著高於未包埋和以1% 藻酸鹽包埋之益生菌的存活率。此外，以1.5% 和 0.4% 幾丁聚醣微膠囊化之益生菌置於25oC 下 60 天後的存活率顯著高於未膠囊化之益生菌。因此，用 0.4% 幾丁聚醣和 1.5% 藻酸鹽包埋B. subtilis E20以配製三種蝦的飼料，包括魚粉基礎飼料（對照組）和添加微膠囊化 B. subtilis E20 107 (En-107), 108 (En-108), 和109 (En-109) CFU kg-1 的飼料。與餵食對照組的蝦相比，餵食107, 108, 和109 CFU kg-1天的蝦具有更明顯的增重、長率和最終體重。同樣的，與對照組和添加107 CFU kg-1 相比，投餵添加108, 和109 CFU kg-1 包埋益生菌的白蝦，飼料效率顯著增加。在成長試驗的過程中每組的蝦死亡數無明顯差別。投餵添加109 CFU kg-1 包埋益生菌飼料的蝦以溶藻弧菌注射感染後的死亡率顯著低於其他組。THC 活性在對照組和107, 108, 109 CFU kg-1 中具有顯著差異。與對照組相比，投餵 109 CFU kg-1 包埋益生菌的蝦PO 活性有顯著差別，但投餵107 CFU kg-1與對照組沒有顯著差異。 SOD和RB的活性沒有顯著差異。 PA與對照組相比具有顯著差異，並且處理之間也顯示出顯著差異，在109 CFU kg-1組中發現了最高值。這些發現表明，與未包埋之益生菌相比，微膠囊化包埋後的益生菌增加了白蝦的活力和抗病性。

關鍵字

枯草芽孢桿菌 ； 微膠囊化益生菌 ； 藻酸鹽 ； 幾丁聚醣

並列摘要

This study aimed to encapsulate the probiotic Bacillus subtilis E20 in alginate and chitosan, assess its viability probiotic in simulated gastric fluid (SGF) pH 2, simulated intestinal fluid (SIF) pH 7, and evaluate the efficiency of coated probiotic B. subtilis E20 at different doses on growth performance and disease resistance of shrimp. B. subtilis E20 coated with 1.5% and 2% alginate had significantly higher survival than non-encapsulated and 1% alginate in SGF and SIF. In addition, the viability of encapsulated probiotics 1.5% and 0.4% chitosan at 25oC was significantly higher than that of non-encapsulated probiotics at 60 days. Therefore, B. subtilis E20 was coated with 0.4% chitosan and 1.5% alginate to formulate three dietary treatments for shrimp, including fish meal-based feed (control) and feed containing encapsulated B. subtilis E20 at level 107 (En-107), 108 (En-108), and 109 (En-109) CFU kg-1. Shrimp fed encapsulated probiotic at level 107, 108, and 109 CFU kg-1 for 56 days had significantly higher final weight, WG, and SGR compared to shrimp fed the control diet. Similarly, FE was significantly increased at the encapsulated probiotic at level 108 and 109 CFU kg-1 compared to the control group and encapsulated probiotic at level 107 CFU kg-1. Shrimp survival between treatments was not significantly different. Shrimp fed encapsulated probiotic feed at level 109 CFU kg-1 showed significantly lower mortality than other treatments after being challenged with Vibrio alginolyticus. THC activity showed significant differences in the control group and encapsulated probiotic at level 107, 108, and 109 CFU kg-1 but not significantly different between treatments. The PO activity was significantly different in the encapsulated probiotic at level 109 CFU kg-1 treatment compared to the control, but the control and encapsulated probiotic 107 CFU kg-1 treatments were not significantly different. There was no significant difference in the activity of SOD and RB. PA showed a significant difference compared to the control, and a significant difference was also shown between the treatments. The highest value was found in the encapsulated probiotic at level 109 CFU kg-1. These findings indicated that encapsulated probiotics increased viability and increased disease resistance of white shrimp compared to the non-encapsulated probiotics.

並列關鍵字

B. subtilis ； Encapsulated probiotic ； Alginate ； Chitosan

參考文獻

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