以超微弱冷光為主軸的探針技術評估六種不同中草藥的抗自由機能力

Detection of the Radical-Scavenging Abilities of six Chinese herbal medicines by Probe-Based Ultraweak Chemiluminescence Technique

陳國維(Kuo-Wei Chen)

指導教授 ： 段國仁

大同大學/工程學院/生物工程學系所/碩士(2006年)

摘要

在全世界己有一股回歸自然，重新面對天然藥物的潮流與需求。中藥，是華人數千年累積的智慧結晶，一個蘊藏了許多抗衰防老，益壽延年的良藥寶庫；金絲桃素、人蔘幾個中草藥的成功，更促使傳統中草藥的研發成為國際間，各國爭相介入與積極開發的市場。因此，以6種自古流傳的中草藥作為本次研究目標，希望能找出其具有抗氣化功能的效果。
在本研究中，使用最新檢測技術；利用同一化學原理使用對氧為中心自由基之啟動系統以及爾後產生超微弱冷光之探針，在同一部機器，能同時偵測多種以氧為中心的自由基：如超氧陰離子（superoxide anion）、羥自由基（hydroxyl radical）及過氧化自由基（peroxyl radical）。藉由此方法，進一步來評估市面上相當受歡迎的6種中草藥的抗氧化能力，包括：1.仙草，2.甘草根，3.銀杏葉，4.橄欖葉，5.綠茶，6.肉桂，並從我們所測到不同的結果，經由計算IC50的數值（抑制50%超微弱冷光所需的濃度），可以比較並排列出這些中草藥萃取物，對於個別自由基的抵抗能力之順序，經分析評估結果，其對不同自由基之清除能力分別為；1)抗超氧化陰離子IC50能力高低，依序為：5.(1.37 μg)> 1.(1.78 μg)> 6.(3.15 μg)> 4.(16.69 μg)> 2.(18.45 μg)> 3.(22.82 μg)， 2) 抗羥基自由基IC50能力高低，依序為：5.(3.36 μg)> 1.(4.15 μg)> 6.(23.82 μg)> 4.(28.63 μg)> 2.(43.53 μg)> 3.(46.71 μg)， 3) 抗過氧化自由基能力高低，依序為：1.(0.18 TEU)> 5.(0.14 TEU)> 6.(0.04 TEU)> 4.(0.03 TEU)> 3.(0.023 TEU)> 2.(0.020 TEU)。同時，可藉所測得結果>在抗氧化作用上的不同功效，實際應用於日常生活中，作為選擇與攝取抗氧化及養生保健品及其調理之參考。

關鍵字：仙草、甘草根、銀杏葉、橄欖葉、綠茶、肉桂、抗氧化、自由基、超微
弱冷光、TEU (Trolox-Equivalent Unit)

關鍵字

超微弱冷光

並列摘要

Our propensity to relate everything back to the natural world has globally initiated an emerging trend to once again focus on nature and its impact on Chinese herbal medicine. The art and science of today’s Chinese herbal medicine is the attribution from thousands of years of ancestral knowledge and practices. In addition, the Chinese apothecary treasures an array of the oldest and most efficacious remedies to combat and treat aging. To name a few, Ginseng and St. John’s wort both are the epitomes of the Chinese herbal medicine, which have slowly but successfully gained their popularity into the global mainstream of today’s pharmaceutical market.
Thus, we will select six legendarily prominent Chinese herbal medicines to be our main research objectives. Our intent for this study is to understand its anti-oxidation chemistry and to find a direct correlation between Chinese herbal medicine and its effect as an antioxidant agent. We used a novel probe-based ultraweak chemiluminescence (uwCL) technique capable of detecting a panel of three oxygen-derived free radicals (ODFRs) including superoxide anion (O2‧-), hydroxyl radical (‧OH) and peroxyl radical (ROO‧).
Our approach is to use this technique to assess the radical-scavenging ability (RSA) of the following six popular Chinese herbal medicine: 1.Mesona chinensis Benth, 2.Licorice, 3. Ginkgo leaf, 4.Olive leaf, 5.Green tea, 6.Cassia bark. By comparing the difference of the IC50 values of each material, we were able to get the sequence of the six Chinese herbal medicine based on their RSA. For 1) O2-.scavenging ability, the order of inhibitory efficiency were: 5.(1.37 μg)> 1.(1.78 μg)> 6.(3.15 μg)> 4.(16.69 μg)> 2.(18.45 μg)> 3.(22.82 μg), respectively; for 2) •OH -scavenging ability, the order of inhibitory efficiency were: 5.(3.36 μg)> 1.(4.15 μg)> 6.(23.82 μg)> 4.(28.63 μg)> 2.(43.53 μg)> 3.(46.71 μg), respectively; for 3) ROO• -scavenging ability were 1.(0.18 TEU)> 5.(0.14 TEU)> 6.(0.04 TEU)> 4.(0.03 TEU)> 3.(0.023 TEU)> 2.(0.020 TEU), respectively. The test results help us know how to choose healthcare products in our daily lives, specifically how to intake the recommended dosage of antioxidants.

Key words—Mesona chinensis Benth, Licorice, Ginkgo leaf, Olive leaf, Green tea, Cassia bark, Anti-oxidation, Free radical, Ultraweak Chemiluminescence (uwCL), TEU (Trolox-Equivalent Unit)

並列關鍵字

Ultraweak Chemiluminescence

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