香椿水萃取物對於酪胺酸酶活性之抑制效能

Inhibition of the Water Extract from Toona sinensis toward the Activity of Tyrosinase

闕壯銘(chiue chuang ming)

指導教授 ： 許成光

亞洲大學/健康學院/生物科技學系碩士班/碩士(2007年)

摘要

在生物體內，當酪氨酸酶分解某些受質，如：L-3,4-dihydroxyphenylalanine (L-dopa)、L-tyrosine可導致麥拉寧黑色素(melanin)的形成。本研究探討香椿葉片的水萃液(TSL-CE)、水萃上清液(TSL-1)及香椿葉發酵液(TSL-1 F)對酪氨酸酶分解L-dopa、L-tyrosine活性的影響，以評估香椿作為皮膚保養品的可能性。本實驗中酪氨酸酶的活性乃以在分光光度計475nm下所偵測dopachrome的生成量為指標。實驗的數據指出無論是TSL-CE、TSL-1及TSL-1 F都會有明顯抑制酪氨酸酶分解L-dopa的作用。此外，抑制作用隨著水萃物濃度(0.047，0.375，1.5，3，6mg/ml) 的濃度升高而增加，在L-dopa的濃度為5mg/ml，TSL-1、TSL-CE、TSL-1 F濃度為6mg/ml狀況下，抑制率分別達到: 85.37%、58.88%以及56.21%。當酪氨酸酶的受質為L-tyrosine時，三種香椿水萃液也有抑制酪氨酸酶的作用，而且抑制作用也隨著水萃物濃度升高而增加，在L-tyrosine的濃度為5mg/ml，TSL-1、TSL-CE、TSL-1 F等的濃度為6mg/ml狀況下，抑制率分別達到: 95.83%、63.61%以及66.69%。比較三種香椿葉片水萃取液，TSL-1對酪氨酸酶的抑制作用為最佳。由動力學的參數得知，TSL-1、TSL-CE、TSL-1 F在受質為L-dopa2以及L-tyrosine時(除了TSL-CE在L-tyrosine當受質的情況下無法判定)，對於酪氨酸酶的抑制形態都為混合型抑制。

關鍵字

香椿 ； 酪氨酸. ； 抑制作用

並列摘要

In living organisms, melanin can be formed by enzymatic reaction of tyrosinase with certain substrates such as L-3,4-dihydroxyphenylalanine (L-dopa) or L-tyrosine. In this study, we determined the effects of water extract from the leave of Toona sinensis (TSL-CE), the supernatant of the water extract (TSL-1) and the supernatant of the water extract from the fermented leaves (TSL-1 F) on the activities of mushroom tyrosinase toward L-dopa or L-tyrosine to evaluate the potential of using Toona sinensis as the source of skin health products. The activity of tyrosinase was measured by determining the formation rate of dopachrome in 475 nm. Our data indicated that TSL-1, TSL-CE and TSL-1 F all showed significant inhibition on the activity of tyrosinase using L-dopa as the substrate. Also, the inhibitory effect increased with the concentrations of the extracts in the range of 0.047-6mg/ml. At the level of 6 mg/ml, TSL-1, TSL-CE and TSL-1 F exhibited 85.37%, 58.88% and 56.21% inhibition, respectively, on the activity of mushroom tyrosinase toward L-dopa (5 mg/ml). The inhibitory effect also took place when L-tyrosine was served as the substrate. At the level of 6 mg/ml, TSL-1, TSL-CE and TSL-1 F exhibited 95.83%, 63.61% and 66.69% inhibition, respectively, on the activity of mushroom tyrosinase toward L-tyrosine. Among three extracts, TSL-1 showed the highest inhibitory effect toward the activity of mushroom tyrosinase. TSL-1 TSL-CE and TSL-1 F showed mixed-type inhibition to mushroom tyrosinase when L-dopa and L-tyrosine were served as the substrates (except it was hard to define the type of inhibition for TSL-CE when L-tyrosine as substrate).

並列關鍵字

Toona sinensis ； Mushroom tyrosinase ； Inhibition effect

參考文獻

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