Pocket formation and alveolar bone loss are common results of periodontitis observed at dental clinic. A goal of periodontal therapy is to obtain periodontal regeneration and then reduce the destruction of periodontal tissue. Recently due to the development of material technology, enamel matrix derivative(EMD, Emdogain®) is now using as a tool to make regeneration of periodontal tissue and so that increase the efficacy of periodontal surgery. These two cases showed treatment results of using EMD in intrabony periodontal defects.