Radioimmunoassay for Ghrelin: Evaluation of Method and the Effect of Fasting in Rats

A double antibody radioimmunoassay (RIA) for ghrelin has been developed and characterized. Antisera against the human ghrelin conjugated to bovine serum albumin (BSA) were produced in the rabbit. Since the ghrelin of human and rat are close homologies, this antiserum (No. YJC 7-36) is cross-reacted (100%) to rat ghrelin. Therefore, it can be applied to both human and rat RIA of ghrelin for measurement of ghrelin in serum. No cross-reactivities with human insulin, rat growth hormone, somatostatin, and glucagon were detected in this RIA system. Very weak cross-reactivity with human growth hormone was found (0.65%). Sensitivity of the RIA was 300 pg per assay tube. The within-assay precision of serum samples were 4.8% in the human being and 8.7% in the rat. The between-assay precision was 9.4% in human being and 9.0% in rats. The serum ghrelin levels of 12 rats were 1.87±0.29 ng/ml in non-fasting status, but 4.28±0.58 ng/ml in fasted rats.

並列關鍵字

anti-ghrelin serum ； ghrelin RIA ； rat serum ghrelin ； human serum ghrelin

被引用紀錄

Chen, Y. P. (2011). 於平面基板與滾筒表面以步進對準式干涉微影技術接合次微米週期性圖案 [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2011.01439

Chen, C. H. (2009). 奈米週期性干涉條紋的接合技術 [doctoral dissertation, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2009.00732

延伸閱讀

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Radioimmunoassay for Ghrelin: Evaluation of Method and the Effect of Fasting in Rats

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