The effect of familiarity on the landscape preference is one of the major issues in environmental perception research. Since tourists are generally supposed to experience things that differ from normal life, it is reasonable to expect that people will prefer unfamiliar landscapes when they are traveling. This study examined the relationship between familiarity and landscape preference in travel and everyday life scenarios. With a photo-based survey, a significant but slight negative relationship was found between familiarity and travel landscape preference, while no significant relationship was found between familiarity and everyday life landscape preference. Discussion and implications were provided in the study.