低能量的低溫噴射式大氣DBD電漿對纖維母細胞影響之研究

Low Energy Non-Thermal Atmospheric-Pressure Dielectric Barrier Discharge Plasma-Jet Treatment of Fibroblasts

張翰倫(Han-Lun Chang) ； 陳貞穎(Chen-Ying Chen) ； 王明誠(Ming-Chen Wang)

《長庚科技學刊》 19期 (2013/12) Pp. 7-17

https://doi.org/10.6192/CGUST.2013.12.19.2

摘要

低溫噴射式大氣電漿被廣泛的應用在醫療上，包含滅菌、傷口癒合以及使惡性腫瘤凋亡等。電漿被證明可以在組織上進行滅菌，但不會使組織發炎，在慢性傷口的治療上，電漿已經成為一種很有吸引力的工具。不同的電漿參數對細胞造成的影響也不同，如造成細胞增生、凋亡或是細胞膜的損傷，低劑量的電漿對血管內皮細胞是相對無毒性，而高劑量的電漿會造成內皮細胞死亡。適當的電漿參數可能會促使纖維母細胞增生，纖維母細胞的增生在傷口癒合的皮膚生物學上產生關鍵作用，本研究評估低溫噴射式大氣電漿對纖維母細胞的細胞活性以及增生能力，我們的目標是優化電漿處理的實驗參數，例如處理時間及外加的功率等，進而促使纖維母細胞增生，治療傷口癒合。最後，我們成功利用電漿促使纖維母細胞增生，其處理參數為處理時間10秒、培養基體積0.4ml以及電漿功率17.47W。

關鍵字

噴射式低溫電漿 ； DBD大氣電漿 ； 纖維母細胞 ； 傷口癒合

並列摘要

Non-thermal atmospheric-pressure dielectric barrier discharge (DBD) plasma-jet is being widely developed for medical applications, including wound healing, malignant cell apoptosis and bacterial sterilization. The plasma process is demonstrated to cause bacterial inactivation and non-inflammatory tissue modification. It has become an attractive tool for improvement in chronic wound management. The parameters of plasma treatment influence the effect of cell, including proliferation, apoptosis and cell membrane damage. Low energy plasma is relatively non-toxic to fibroblasts while treatment at longer exposures led to cell death. Appropriate parameters of treatment will enhance cell proliferation. Proliferation of fibroblasts is a key role about wound healing in skin biology. In this study, we investigate the proliferation/toxicity of non-thermal atmospheric-pressure helium DBD plasma-jet treatment on fibroblasts. Our goal is to optimize plasma treatment by varying experimental parameters, such as treatment time and applied power, to enhance proliferation of fibroblasts for chronic wound healing. Finally, we get the optimization of plasma parameters (the treatment time is 10 s, the liquid volume is 0.4 ml, and the applied power is 17.47 W) for cell proliferation.

並列關鍵字

non-thermal plasma-jet ； dielectric barrier discharge (DBD) ； fibroblasts ； wound healing

參考文獻

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