由於左旋維他命C本身不安定且易衰減,因此如何延長左旋維他命C的活姓是極重要的課題。本研究的目的在嘗試利用蒙脫石來提升左旋維他命C的安定性。另外,也以低毒性的磷酸配製不同酸處理程度的蒙脫石來ㄧ同比較安定左旋維他命C的功用。並以XRD (X-ray diffraction)、EDS(Energy Dispersive X-ray Spectrometer)、BET分析儀來探討改質後蒙脫石的性質變化。經由UV光譜的定量分析顯示,在左旋維他命C的水溶液中加入蒙脫石確實使其衰減速率有大幅降低的效果(純左旋維他命C在 12小時衰減近95%;加入適當濃度(0.1%w/v)蒙脫石後,左旋維他命C在 12小時衰減28%),而改質後的蒙脫石(0.01N酸處理)則以加入較蒙脫石少的量就可達相同的穩定左旋維他命C效果。 且經由微生物毒性測試顯示蒙脫石與改質後的蒙脫石為安全無毒性材料。並於人體皮膚纖維母細胞活性測試發現濃度為10%w/v的左旋維他命C溶液有極佳的刺激細胞活性增加效果(較無添加左旋維他命C溶液的細胞,其OD測試值(optical density value)高了約11倍),且實驗發現加入蒙脫石或酸改質蒙脫石並不會對細胞活性有明顯不良的影響。因此根據目前的實驗數據顯示,利用蒙脫石來安定左旋維他命C應為一可行之方法。
As L-ascorbic acid (LAA) is unstable in nature. This study is trying to use montmorillonite to provide feasible strategy to stabilize LAA for practical uses. In addition, using acid treated montmorillonite (MMT) to compare the effect of stabilizing LAA that formulate by low toxic phosphoric acid. To analyze the property changed of modified MMT by XRD (X-ray diffraction)、EDS(Energy Dispersive X-ray Spectrometer)、BET analyzer. According to Single-beam scanning UV/VIS spectrophotometer showed that the decomposition rate of LAA was decreased by adding MMT (In 12 hours, the LAA (0.001%w/v) delayed 97% of activity; In contract, LAA mixed with MMT (0.1%w/v) will only decompose 28%.). In addition, adding less amount of modified MMT (0.01 N acid treated) than MMT could had the same effect of stabilizing LAA. Toxicity assessment also showed that MMT treated with low level acids should be safe according to our assessment. The test of dermal fibroblast proliferation showed that the 10 %w/v LAA could assist fibroblast proliferation (The optical density value was increased 11 fold). The experiment of dermal fibroblast proliferation also appeared that adding MMT or acid treated MMT would not had harmful effect on dermal fibroblast. Thus, using MMT or acid treated MMT to stabilize LAA might be a feasible method of LAA for practical uses.