本研究主要為分析化妝品常用之二十種精油對於清除ABTS自由基(2,2'-azino-bis〔3-ethylbenz thiazoline-6-sulphonic acid〕)與其主要之化學成分之分析。結果顯示二十種試驗樣品中以丁香精油(Clove bud essential oil)與紅百里香精油(Thyme red essential oil)清除ABTS自由基之能力最為顯著。當丁香精油與紅百里香精油樣品濃度為1mg/mL時,分別能清除96.33%及95.98%之ABTS自由基,半數有效抑制濃度(EC_(50))分別為10μg/mL及18μg/mL,以氣相層析質譜儀鑑定其主要之化學成分,發現丁香精油中含有82.32%的丁香酚(Eugenol),而紅百里香精油含有63.665%的百里香酚(Thymol)。綜合以上之結果,證明精油中酚類化合物的含量與清除ABTS自由基之抗氧化能力成正相關係。
Twenty kinds of commonly used essential oils were employed to investigate the ABTS free radical (2,2'-azino-bis [ 3-ethylbenzthiazoline-6-sulphonic acid ] ) scavenging ability and analysis of the major chemical compositions. The free radical scavenging ability of Clove bud and Thyme red are the best two in these essential oils. 1mg/mL of Clove bud essential oil and Thyme red essential oil were shown 96.33% and 95.98% of the ABTS free radical scavenging ability respectively. Their EC_(50) are 10μg/mL and 18μ g/mL. 82.32% of eugenol in Clove bud essential oil and 64.25% of thymol in Thyme red essential oil were analyzed by GC-MS. It is clear that the phenol compounds in essential oils and the ABTS free radical scavenging ability is in direct proportion.