都市污水中醣類、蛋白質及脂質定性定量分析研究

QUALITATIVE AND QUANTITATIVE ANALYSIS OF SUGAR, PROTEINS AND LIPIDS IN DOMESTIC SEWAGE

邱明祺(Ming-Chi Chiu) ； 陳昶瑞(Chang-Jui Chen) ； 陳虹瑤(Haon-Yao Chen) ； 陳子揚(Tzu-Yang Chen) ； 李建賢(Chien-Hsien Lee) ； 林正芳(Cheng-Fang Lin)

《中國土木水利工程學刊》 32卷1期 (2020/03) Pp. 91-99

https://doi.org/10.6652/JoCICHE.202003_32(1).0008

摘要

都市污水以厭氧生物處理可以產生甲烷，讓都市污水處理廠轉換為產能而非耗能的綠色污水處理廠，然厭氧生物處理都市污水有其難度，尤其如何在短暫的水力停留時間將污水中的醣類、蛋白質及脂質水解、酸化及甲烷化，本研究係探討都市生活污水中醣類、蛋白質及脂質三基質成分檢測方法，以利研究短暫水力停留時間厭氧生物處理都市污水之醣類、蛋白質及脂質成分之基質轉換程度探討，作為厭氧生物處理都市污水之先驅研究；醣類使用「蔥酮法」，蛋白質使用「莫瑞法」，脂質使用「紅外線分析法」，經實驗各方法之定量極限（LOQ）與偵測極限（LOD）均能有效檢測都市污水各成分範圍，此外在實際都市污水添加試驗證實本研究分析方法較無基質消耗與干擾等問題存在，且在複雜的混合水體中應用上具檢測可行性。另依前述方法實測國內夏季期間污水處理廠都市污水結果顯示，我國都市污水基質成分脂質最高，蛋白質次之，醣類為最低，此基質分布結果可作為後續厭氧生物處理研究降解機制之參考。

關鍵字

厭氧生物處理 ； 都市污水 ； 醣類 ； 蛋白質 ； 脂質

並列摘要

Using anaerobic biological method in treating domestic sewage can generate methane so that the domestic wastewater treatment plant can be an energy yield facility but not energy sink plant. However, it is very difficulty to anaerobically treat domestic sewage in very short hydraulic detection time due to the hydrolysis, acidogenesis and methanogenesis steps for sugar, protein and lipid. To fully understanding the bio-transformation of domestic sewage, it is very important to quantify and qualitify these three components in domestic sewage. This study assesses the methods that are appropriate in quantifing and qualitifing the sugar, protein and lipid component in domestic sewage. This study has shown that Anthrone method can be used on carbohydrates, Lowry method can be used on proteins, and Infrared Spectrometric method can be used on fat. The quantitation limit (LOQ) and the detection limit (LOD) of each method can detect the range of urban wastewater effectively. Additionally, the actual urban sewage test confirms there is no problem such as substrate consumption and interference in the research method. It is feasible for the application of urban wastewater. With these methods, the percentages of organic components in the wastewater were defined. Lipid is the highest and sugar is the lowest ratio in domestic wastewater. The results of sugar, protein and lipid distribution can be used as a basis for follow-up study on the degradation mechanism of anaerobic biodegradation.

並列關鍵字

anaerobic treatment ； domestic wastewater ； carbohydrate ； protein ； lipid