Grouper culture is an important source of income in Viet Nam. However, during the last decade, the industry has suffered numerous serious disease outbreaks, including some due to pathogenic parasites. This study examined the prevalence and intensity of Caligus species on two commonly cultured Vietnamese groupers, Epinephelus coioides and E. bleekeri. A total of 469 fish (241 Epinephelus coioides, 228 E. bleekeri) from both brackish and marine waters, including wild, pond cultured and cage cultured fish, were examined. Two caligid species, Caligus epidemicus and C. multispinosus, were found. In addition, caligid larvae were found on skin and/or gills. Differences in parasite burden were found between host species and within host species by environment.