分子標誌應用於彩色甜椒遺傳差異性之評估

Assessing the Genetic Variation of Capsicum annuum Based on Molecular Markers

王昭月(Jau-Yueh Wang) ； 曾夢蛟(Menq-Jiau Tseng)

《植物種苗》 10卷2期 (2008/06) Pp. 32-54

https://doi.org/10.30077/SN.200806.0003

摘要

利用分子標誌評估作物遺傳差異性，有助於系統性選種效率之提升。本研究利用random amplified polymorphic DNA（RAPD）、inter simplesequence repeat（ISSR）與simple sequence repeat（SSR）三種分子標誌進行番椒（Capsicum annuum）36品系之遺傳歧異性分析。試驗結果顯示，自26個RAPD引子之PCR反應獲得302個核酸片段產物，其中233個核酸片段具多型性（polymorphism 77.2%）；自10個ISSR引子之PCR反應獲得160個核酸片段，其中142個核酸片段具多型性 （polymorphism 88.8%）；自99對SSR引子組篩選出12對具多型性，PCR反應產物為61個alleles，平均多型性訊息含量（polymorphic information content，PIC）0.569。合併RAPD、ISSR與ISSR分子標誌進行UPGMA群集分析，結果顯示不同群集可對應出不同番椒果形差異；而合併RAPD、ISSR與SSR等三種分子標誌PCR資訊之UPGMA群集分析的遺傳關係結果顯示，不同群集可對應不同果形，且針對夏季產量較高之品系亦可區別。以上三種分子標誌再以二維主成份分析（2D principle component analysis，2D-PCA）分析結果，夏季產量較高之品系，亦可獲得標定。綜合UPGMA與PCA分析結果顯示，合併多種分子標誌之分析可提供較多的多型性標誌，有利於相近品種間之遺傳分析，可作為選種材料遺傳背景之評估。

關鍵字

番椒 ； 分子標誌 ； 遺傳相似性 ； 群集分析

並列摘要

Assessment of the genetic diversity by molecular markers is useful for their systematic and high efficiency of the breeding program. There were 36 genotypes of Capsicum, including 34 genotypes of sweet pepper and 2 genotypes of chili pepper, which were conducted by random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) to estimate pair-wise genetic similarity. Results showed that 302 RAPD fragments could be generated from 12 primers, and 233 with polymorphism (77.2%). For ISSR analysis, 160 fragments were obtained from 10 primers, and 142 with polymorphism (88.8%). For SSR assay, 12 SSR pairs were surveyed from 99 SSR pairs which generated 61 alleles and the polymorphic information content (PIC) was 0.569. Furthermore, a combination of RAPD, ISSR and SSR markers assessed the cluster analysis of UPGMA, showed the relationships among groups following the fruit shapes. While combined all of the RAPD, ISSR and SSR markers also revealed the groups generally cluster together with similar fruit shapes and the high yield genotypes in summer could be distinguished too. Combining these three marker systems and assessed principle component analysis (PCA) revealed that the genotypes with higher yield in summer could be distinguished clearly. The analysis of UPGMA and PCA, suggested that a combination of different kinds of markers could provide sufficient polymorphism to assess genetic variation even within closely related species.

並列關鍵字

Capsicum ； molecular markers ； genetic similarity ； cluster analysis