農桿根群菌促進台灣紅豆杉成熟樹插穗發根

Enhancement of Agrobacterium rhizogenes on Root Formation of Taxus mairei Cuttings of Mature Trees

陳國峰(Kao-Phone Chen) ； 何政坤(Cheng-Kuen Ho) ； 郭幸榮(Shing-Rong Kuo)

《台灣林業科學》 18卷3期 (2003/09) Pp. 213-223

https://doi.org/10.7075/TJFS.200309.0213

摘要

為促進台灣紅豆杉插穗發根，本研究採集4株成熟樹分別在1998年12月31日與1999年10月23日進行二次農桿根群菌接種插穗的扦插試驗。第一次試驗結果顯示農桿根群菌種R15834對4單株插穗發根促進較佳。第二次試驗增加在插穗切口處上方1.5cm的莖基處以刀片縱割1cm的傷口，以增加感染面積；在培養菌液添加acetyrigone、proline與betaine等增加細菌感染能力的添加劑。結果發現接種農桿根群菌對易發根與難發根插穗刀傷處的發根率各為42.2與0.3%，而未接種插穗的刀傷處都未發根。推測此一刀傷發根率反應出農桿根群菌的基因轉殖率。不過我們發現增加刀傷的插穗發根率與未刀傷插穗的發根率並未呈現顯著差異，此表示插穗不需要增加新的傷口，原插穗切口就足以當作農桿根群菌的感染傷口。對易發根插穗，農桿根群菌處理比對照組可縮短插穗抒插30d及增加3倍的發根數。難發根插穗需要較長的抒插時間，將扦插時間由120d增加到150d，比較對照組，菌液添加acetosyrigone與proline可顯著增加難發根插穗的發根率6倍以上，顯示難發根插穗的轉殖過程與不定根形成需要較長的時間。

關鍵字

農桿根群菌 ； 成熟樹 ； 台灣紅豆杉 ； 扦插

並列摘要

To increase the adventitious root formation of Taxus mairei cuttings collected from 4mature trees, 2cutting tests using treatment with Agrobacterium rhizogenes were conducted on 31 Dec 1998 and 23 Oct 1999, respectively. The first test showed that treaking cuttings with Agrobacterium R15834 was effective in increasing the rooting percentages and numbers. In the second test, a 1 cm-long wound was made on basal parts 1.5 cm above the cut surfaces using a surgical blade to in crease the trans formation area. Bacterium medium supplement with acetosyrig one, praline, and betaine to increase the infection efficiency was also tested. Rooting percentages induced from the wounds of bacterium-treated cuttings for easy-and hard-to-root trees were 42.2 and 0.3%, respectively, whereas no roots were found in wounds of cuttings which were not treated with bacterium. We presume that these percentages can be represented as trans formation rates. However, we also found that the rooting percentages of wounded cuttings did not differ from these of unwounded ones. This means that creating additional wounds by cuttings is not necessary, and the cut surfaces are sufficient for Agrobacterium infection. Agrobacterium-treatment of cuttings from easy-to-root trees shortened the cutting period by 1 month and in creased the rooting numbers by 3 times compared to control cuttings after cutting for 120 d. For hard-to-root cuttings, a longer cutting period was required. By increaseing the cutting period to 150 d and adding acetosyri gone and praline to the bacterium medium, the rooting percentage of treated cuttings increased 6 times compared to untreated ones, indicating that both the trans formation process and adventitious root formation in hard-to-root cuttings require longer time to function.

並列關鍵字

Agrobacterium rhizogenes ； cutting ； mature trees ； Taxus mairei