利用相位相關與二值化配準多功能掃描式顯微鏡在不同掃描方式下所造成的影像差異

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Cellege of Optoelectronics > Graduate Institute of Lighting and Energy Photonics
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利用相位相關與二值化配準多功能掃描式顯微鏡在不同掃描方式下所造成的影像差異

Phase Correlation and Binary Threshold Register for Alignment and Correction of Multifunctional Scanning Microscope Images Obtained Using Different Scanning Methods

吳旻哲 , Masters　　Advisor：劉佳明　　

繁體中文

共焦式掃描顯微鏡；光學顯微鏡；影像處理；相位相關法；二值化；影像配準； Confocal scanning microscope ； Optical microscope ； Image processing ； Phase correlation ； Binary threshold ； Image registration

Abstract │ Reference (22) │ Altmetrics

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We construct a multifunctional scanning microscope system. There are two scanning methods: One scans by moving a nanopositioning stage, and another by swaying galvanometer mirrors. Because of the optical path difference of two scanning method, there are differences in translation, rotation, and scaling between two images. In order to make the multifunctional scanning microscope system practically useful, we developed image registration technique to accurately calibrate the images obtained using the two scanning methods.
Phase correlation cannot be estimated by images having translation, rotation, and scaling differences at the same time. We first use threshold binary image processing to increase accuracy. Then, we overlap the feature points of two images so that the feature points of the nanopositioning stage image could be viewed as the reference rotational axis. The translation, rotation, and scaling parameters are found by phase correlation. Five sets of experiments are carried out to verify that this method can correctly register the relationship of images that are obtained using different scanning methods.

Reference ( 22 ) 〈TOP〉

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