蘋果汁中PATULIN分析方法之探討（一）－薄層層析法與氣相層析法的比較

Studies on the Methodology of Patulin Analysis in Apple Juice-Comparison of the Thin Layer Chromatography and Gas Chromatography

許輔(Fuu Sheu) ； 徐源泰(Yuan-Tay Shyu)

《中國園藝》 41卷2期 (1995/06) Pp. 127-135

https://doi.org/10.6964/JCSHS.199506.0127

摘要

本實驗探討以薄層層析法(TLC)及氣相層析質譜法(GC/MS)分析蘋果汁中patulin含量之優劣。結果顯示薄層層析法之偵測極限(100μg/l)高於patulin的安全限量(50μg/l)，不適用於定量patulin。將patulin衍生爲patulin acetate再配合GC分析及比對檢量線，可準確定量，回收率90%，偵測極限10μg/l。實驗亦發現若直接於樣本中進行醯化衍生反應(acylation derivation)可提高回收率。

關鍵字

薄層層析 ； 氣相層析 ； 衍生化 ； 醯化

並列摘要

Analytical methodology for patulin contents in apple juice was studied by the comparison of thin layer chromatography (TLC) and gas chromatography with moss spectrometry (GC/MS). Results indicated that the determination limit of TLC (100μg/l) was Shove the safe contamination level (50μg/l) of patulin. A method for the analysts of patulin in apple juice was developed using GC of patulin acetate. Patulin acetate can be measured down to a level of 10μg/l with 90% recovery. Results also indicated that conversion of patulin to patulin acetate in aqueous samples before extraction can increase the extinction and recovery efficiency.

並列關鍵字

patulin ； thin layer chromatography ； gas chromatography ； derivation ； acylation