食用油脂中多環芳香族碳氫化合物多重殘留檢驗方法之建立

The Study on the Multiresidue Analytical Method for Determining PAHs in Edible Oil

許哲綸(Che-Lun Hsu) ； 於柏伸(Bo-Shen Wo) ； 蘇淑珠(Shu-Chu Su) ； 施養志(Yang-Chih Shih)

《藥物食品檢驗局調查研究年報》 26號 (2008/12) Pp. 183-197

https://doi.org/10.6946/ASRNL.200812.0183

摘要

食品在加熱、烘乾及煙燻過程中會產生多環芳香族碳氫化合物(polycyclic aromatic hydrocarbons, PAHs)，部分PAHs已証實可能具致癌性，其中苯芘(Benzo [a] pyrene)可作為食品中具可能致癌性PAHs存在及其效應之指標。歐盟執委會208/2005號規範特定食品中苯芘最大限量，其中食用油脂為2.0μg/kg。本研究以HPLC建立食品油脂中14種PAHs含量之檢驗方法。食用油脂以isohexane/t-butyldimethylether(95:5, v/v)溶解後，經HR-P固相萃取匣淨化，最後以高效液相層析儀附螢光檢出器進行PAHs含量之檢測。高效液相層析部份，係以Supelcosil LC-PAH層析管柱進行分離，以乙腈及水為移動相進行梯度沖提，於螢光檢出器激發波長290~314 nm及放射波長398~511 nm偵測。14種PAHs之標準曲線判定係數皆大於0.999，於食用油脂中添加5μg/kg之PAHs，其回收率介於60~106%，檢出限量為0.04~3μg/kg，定量極限為0.2~10μg/kg。應用本研究所建立之方法檢測市售食用油脂橄欖油等20件，結果有16件檢體檢出含有PAHs，13件檢出苯芘，含量範圍為0.20~0.73μg/kg，均低於歐盟規範油脂之最大限量2.0μg/kg，顯示無安全顧慮。

關鍵字

食用油脂 ； 多環芳香族碳氫化合物 ； 苯芘 ； 高效液相層析儀

並列摘要

Polycyclic aromatic hydrocarbons (PAHs) form during food processing or cooking. Some PAHs were considered as carcinogens. Benzo (a) pyrene was used as a marker for the occurrence and effect of carcinogenic PAH. In Commission Regulation (EC) No 208/2005, maximun level of benzo (a) pyrene in edible oil was 2.0μg/ kg.
A method to analyze the contents of 14 PAHs in edible oil was developed in this study. Benzo (a) pyrene was extracted from edible oils using isohexane/t-butyldimethylether (95:5, v/v) and cleaned up with HR-P cartridge. The extracts were analyzed by high performance liquid chromatography using with fluorescence detector (HPLC/FL). The Supelcosil LC-PAH column was used for the separation in HPLC. The LC was affected with a gradient elution using acetonitrile and water as mobile phase. Excitation and emission wavelengths of the detector were 290-314 nm and 398-511 nm, respectively. All the linear regression coefficients standard curves of PAHs were higher than 0.999. The recoveries of PAHs (5μg/mL) spiked in edible oil were between 60 - 106%. The limit of detection ranged 0.04-3μg/kg and the limit of quantification was 0.2-10μg/kg. This method was well qualified for the performance criteria of analysis that is requested by EC. The 13 items of the benzo (a) pyrene were detected in 20 various edible oils with a concentration range of 0.20-0.73μg/kg.

並列關鍵字

edible oil ； PAHs ； benzo a pyrene ； HPLC/FL