Effects of Shugan-Huayu Powder, a Traditional Chinese Medicine, on Hepatic Fibrosis in Rat Model

Ching-Chang Cheng ； Nai-Nu Lin ； Yen-Feng Lee ； Lung-Yuan Wu ； Hsiao-Ping Hsu ； Wen-Jane Lee ； Kwong-Chung Tung ； Yung-Tsung Chiu

《The Chinese Journal of Physiology》 53卷4期 (2010/08) Pp. 223-233

https://doi.org/10.4077/CJP.2010.AMK050

並列摘要

Shugan-Huayu powder (SHP) has been administered to outpatients with chronic liver disease without clear anti-fibrosis mechanism. To investigate the anti-fibrosis effects of SHP on liver fibrosis in a rat model and in hepatic stellate cells (HSCs) in vitro, rats were gavaged with CCl4 at 1.0g/kg body weight twice a week for 8 weeks to induce liver fibrosis and the rats were randomly assigned to one of the three groups: -CCl4 alone, low-dose SHP and high-dose SHP. SHP was given by gavages 5 times a week for 8 weeks. Serum, livers and HSCs were assayed for serology, pathology, western blot, zymography and quantitative RT-PCR. Hepatic function improved as decreased serum aspartate aminotransferase and alanine aminotransferase, and collagen deposition and active HSCs were significantly reduced in CCl4-induced liver by SHP treatment. The expression of matrix metalloproteinase-2 (MMP-2) and transforming growth factor-β1 (TGF-β1) mRNA in fibrotic liver showed significant downregulationafter SHP treatment. In vitro, inhibition of α-smooth muscle actin (α-SMA) expression and MMP-2 secretion of active HSCs were also noticed by SHP treatment. SHP has an antifibrotic effect on CCl4-induced liver fibrosis in rats. Anti-fibrotic mechanisms were probably inhibiting activation of HSCs and decreased expression of MMP-2 and TGF-β1.

並列關鍵字

liver fibrosis ； traditional Chinese herb ； hepatic stellate cell ； animal model

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