不同SPME纖維對八種精油指標成份萃取效能之比較

Extraction Efficiency of Eight Major Components in Essential Oils by Different Solid-phase Microextraction Fibers

李曉音(Hsiao-Yin Li) ； 蔡婉雯(Wan-Wen Tsai) ； 徐照程(Jaw-Cherng Hsu)

《弘光學報》 55期 (2009/04) Pp. 193-207

https://doi.org/10.6615/HAR.200904.55.15

摘要

本研究探討頂空固相微萃取法(head-space solid-phase microextraction，HS-SPME)配合氣相層析質譜儀(GC-MS)於分析精油組成時之最適化條件，比較CAR / PDMS與CW / DVB兩種吸附纖維，對八種精油指標成份混合物：limonene、cineole、linalool、linalyl acetate、citral、thymol、caryophyllene與sclareol之吸附特性。探討各項吸附參數的影響，發現以預熱時間10分鐘、精油水浴溫度50℃、吸附10分鐘、脫附5分鐘等條件能獲得最佳之萃取效率。由GC-MS結果比對，非極性CAR / PDMS混合型纖維的均衡吸附特性能涵蓋不同極性之精油成份，與直接注入精油的組成份佈較為相近。適合作為精油及植物揮發性成份頂空萃取的纖維。極性的CW / DVB混合型纖維，則偏向萃取低沸點成份，對於高沸點成份的萃取效率則較差。比較兩SPME纖維的萃取再現性，CAR / PDMS纖維也優於CW / DVB纖維。選擇CAR / PDMS纖維配合本HS-SPME系統條件將能真實呈現植物香氣的原始組成，未來建立精油品質比對資料庫可作為萃取方法最佳操作條件的評估依據。

關鍵字

植物揮發性成分 ； 頂空萃取 ； 固相微萃取 ； 萃取纖維 ； 氣相層析質譜儀

並列摘要

Headspace sampling is an important technique to characterize the volatile fraction of aromatic plants. Combined headspace solid-phase microextraction sampling (HS-SPME) and GC-MS for screening an ideal SPME fiber of plant volitiles composition was evaluated by our studies. A 1.25% essential oil standard solution of limonene, cineole, linalool, linalyl acetate, citral, thymol, caryophyllene and sclareol was prepared and as characteristic markers, which represents eight major classes of volitiles in aromatic plants. The performance of two commercially available SPME fibers were compared to evaluate the head space recovery of eight characteristic components with different polarities, and also to compare their abundance with that obtained by direct injection of standard solution in GC-MS. Pre-heat time, temperature of thermostatic bath, sampling time and desorption time were also evaluated. The results showed that CAR/ PDMS fiber, consisting a porous solid (Carboxen) and a liquid non-polar coating (polydimethylsiloxane), provide more equilibrium adsorption property than CW/DVB fiber. The results proved that HS-CAR/ PDMS-SPME system can successfully be used for the quality assessment of the extraction process and products in essential oils industry.

並列關鍵字

Plant volatiles ； Head space ； Solid-phase microextraction ； SPME fiber ； GC-MS

參考文獻

Arthur C. L. and Pawliszyn J., 1990, Solid phase microextraction with thermal desorption using fused silica optical fibers. Anal. Chem., 62(19), 2145-2148.

Pawliszyn. J., 1997, Solid phase microextraction : theory and practice, Wiley-VCH, New York, 1997.

György, V. and Vékey K., 2004, Solid-phase microextraction: a powerful sample preparation tool prior to mass spectrometric analysis. J. Mass Spectrometry, 39(3), 233-254.

蔡東湖、陳介甫, 1997, 芳香療法, 中醫藥雜誌, 8(1), 23-32.

Deng C., Li N. and Zhang X., 2004, Rapid determination of essential oil in Acorus tatarinowii Schott. by pressurized hot water extraction followed by solid-phase microextraction and gas chromatography-mass spectrometry. J. Chromatogr. A, 1059(1-2), 149-155.