電紡明膠奈米纖維結合積雪草於皮膚組織工程之應用與評估

Application and Evaluation of Electrospinning Gelatin Nanofiber Combined with Centella asiatica for Skin Tissue Engineering

葉人與(Jen-Yu Yeh)

指導教授 ： 樓靜文

中臺科技大學/健康科學院/醫學工程暨材料研究所/碩士(2010年)

摘要

本研究目的為利用靜電紡絲技術進行合適敷材之製備，藉由電紡技術製備出的明膠/聚乙烯醇結合積雪草活性成分之奈米纖維薄膜具有模仿細胞外基質的功能，經由實驗證明纖維母細胞在此結構中具有良好的增生率與貼附性。在材料製備時將最佳濃度之積雪草萃取液(156 μg/ml)參混入明膠/聚乙烯醇溶液中，以靜電紡絲條件-工作電壓：25 kV；工作流速：0.01 ml/h；工作距離：10 cm；工作時間：4 h，將含積雪草萃取液之明膠奈米薄膜紡在具有抑菌功能的幾丁聚醣表面，並與50%戊二醛進行蒸氣交聯45分鐘達到理想的藥物釋放速率，使此奈米結構將積雪草活性成分-積雪草苷緩慢釋放至受傷的皮膚組織中促進皮膚內纖維母細胞增生，且明膠為天然之膠原蛋白熱變之產物，與皮膚接觸不易產生排斥性，隨後會被體液所分解，使此敷材在幫助傷口修復完全時也能同時被組織降解後吸收，避免敷材在撕下時造成傷口的二度傷害。期望此新型敷材能完全符合傷口治療的臨床應用，具有促進傷口癒合、縮短治癒時間的效果，以減輕患者所承受的痛苦。

關鍵字

纖維母細胞 ； 聚乙烯醇 ； 明膠 ； 積雪草 ； 靜電紡絲

並列摘要

The objective of this study is to prepare Gelatin/PVA/Asiaticoside nanofibers via electrospinning as a novel membrane. Gelatin nanofiber added with the active component of Centella asiatica through electrospinning technique was used to simulate as an extracellular matrix. From the cell co-culture experiments including MTT assay, light microscopy observation, it is found that the structure of nanofiber could enhance cell adhesion and proliferation.
The optimal concentration of Centella asiatica extract at 156 μg/ml was added into Gelatin/PVA solution during the fabrication process and spun to the surface of chitosan through electrospinning technique. It was then crosslinked with 50% glutaraldehyde vapor for 45 min to control the drug release in an ideal rate. This result is to ensure that the active ingredients of Centella asiatica-asiaticoside in the nano structure of membrane can be released to the injured skin tissue in an adaptive rate to promote the proliferation of fibroblast.
The new developed wound dressing biomaterial shows good effects in the wound healing either in the promoting way or in the recovering time.The aforementioned study displays that the novel membrane can be used in the clinical treatment and application in the future.

並列關鍵字

gelatin ； PVA ； Centella asiatica ； electrospinning ； fi

參考文獻

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