The service personnel loads higher work pressure and negative emotion. Hence, it is supposed to result in physical and mental load without negative emotion self-management or resource supporting in time and then affects the follow-up working behaviors. The study was applied the JD-R model to explore the impact of customer incivility on the emotional exhaustion and the moderating role of perceived supervisor support. Data was collected from the 660 valid questionnaires of the the first-line service personnel those who work in two domestic commercial banks. The results after hierarchical regression analysis show that the customer incivility had a significant positive effect on the emotional exhaustion, the supervisor support had a significant negative effect on the emotional exhaustion, and the perceived supervisor support had no significant moderating effect on the relation between customer incivility and emotional exhaustion. Both theoretical and practical implications are discussed.