This research, using the Grad Hotel, Taipei as a case study, is to study the relationship between job stress, professional burnout, and psycho-physical health. Using judgmental sampling 469 usable questionnaires were collected. The research findings are: (1) The hotel service employees with different demographic backgrounds are significantly different in their job stress, professional burnout, and psycho-physical health; (2) job stress and professional burnout are significantly co-related; (3) job stress and psycho-physical health are significantly co-related; and (4) job related burnout and psycho-physical health are significantly co-related. Findings can be used in developing criteria forhiring, in training and in management of employees.